EFFECTS OF CHRONIC HIGH FLUORIDE ON ENAMEL DEVELOPMENT

长期高氟对牙釉质发育的影响

• 批准号: 3461992
• 负责人: Pamela K Den Besten
• 金额: $ 10.49万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3461992
• 关键词: ameloblasts; aminoacid; calcium metabolism; dental development; enamel organ; fluoridation; fluorosis; ingested fluoride therapy; tooth enamel; topical fluoride therapy; water supply

Fluorosed dental enamel is caused by the ingestion of above optimal levels of fluoride. Clinically, mild enamel fluorosis appears as white opacities on the teeth, while severe fluorosis results in pitting and loss of surface enamel. These fluorotic teeth are prone to fracture and show abnormal wear patterns which lead to difficult problems in restoration. Fluorosed enamel is characterized by an increase in the content of organic material and a decrease in the mineral. This may be due to a direct effect on the ameloblasts and/or a direct effect on the enamel. The purpose of this proposal is to investigate the effects of the ingestion of chronic high levels of fluoride in drinking water on enamel formation and the mechanisms by which fluorosis occurs. The specific aims include the following: To determine the effects of chronic, high levels of fluoride in the drinking water: (1) on the two classes of proteins in developing enamel; (2) on the cyclic modulation of the maturation ameloblasts as reflected by the staining of the enamel for free and loosely bound calcium; (3) on the morphology of the enamel organ; and (4) to determine the reversibility of the effects of the ingestion of chronic high levels of fluoride in the drinking water on enamel and on the enamel organ. Fluorosed and control enamel proteins will be compared by molecular weight and by amino acid analysis. The modulation of the maturation ameloblasts will be compared histologically using SEM and indirectly by calcein staining of the periodic stripes in maturation enamel. Changes which occur in fluorosed enamel will be compared to the changes which occur in the fluorosed enamel organ. These studies will show when in enamel development high levels of fluoride exert their effect and on which components this effect is manifest, and will lead to further understanding of the mechanisms involved in formation of fluorotic enamel.

氟牙釉质是由摄入超过最佳水平引起的 氟化物。 临床上，轻度氟牙釉质症表现为白色混浊 而严重的氟中毒会导致表面凹陷和丧失 搪瓷。 这些氟斑牙容易发生断裂，表现出异常磨损 导致修复困难的模式。 氟化搪瓷的特点是有机物含量增加， 材料和减少的矿物。这可能是由于直接影响 对成釉细胞和/或对釉质的直接影响。 的目的 这项建议是调查摄入慢性 饮用水中高含量的氟化物对牙釉质形成的影响， 氟中毒发生的机制。 具体目标包括： 慢性、高氟饮水：（1）对两个 类蛋白质在发育中的釉质;（2）对周期性调制 成熟的成釉细胞，如通过釉质染色所反映的， （3）对成釉器形态的影响; 以及（4）确定摄入以下物质的影响的可逆性： 饮用水中的慢性高水平氟化物对牙釉质和牙釉质的影响 釉器 将通过分子量比较氟化和对照釉质蛋白 和氨基酸分析。 成釉细胞成熟的调控 将使用SEM和钙黄绿素间接进行组织学比较 在成熟的釉质中周期性条纹的染色。 发生的变化 将氟牙釉质中的变化与氟牙釉质中的变化进行比较， 氟化釉质器官 这些研究将表明，当在牙釉质发育高水平的氟 发挥它们的作用，以及这种作用在哪些成分上表现出来， 将导致进一步了解参与形成的机制， 氟斑牙釉质