ROLE OF OXIDANTS IN ACUTE INFLAMMATORY INJURY

氧化剂在急性炎症损伤中的作用

• 批准号: 3455034
• 负责人: INGRID U SCHRAUFSTATTER
• 金额: $ 8.45万
• 依托单位: SCRIPPS CLINIC AND RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1994-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3455034
• 关键词: DNA footprinting; DNA repair; cell morphology; chlorine; human tissue; hydrogen peroxide; inflammation; laboratory mouse; laboratory rabbit; lymphocyte; macrophage; membrane permeability; neutrophil; oxidizing agents; radiotracer; tissue /cell culture

We and others have observed that oxidants are formed during inflammatory tissue injury and are known to be generated through stimulation of leukocytes. In order to evaluate the role of oxidants in the pathogenicity of inflammatory disease, it will be essential to define the cellular and molecular targets of oxidants. The applicant proposes to study the effect of specific oxidants on molecular targets of cells. In particular, the proposed studies are aimed at: 1.0 Definition of cellular targets of H202 1.1 Definition of cellular targets of HOCl and chloramines 1.2 Determination of the effects of oxidants derived from stimulated PMNs on target cells with special emphasis on damage of DNA. Once specific targets of oxidants are defined on a molecular level, it will be possible to determine oxidant involvement in inflammatory disease by looking for the formation of these "footprints" in vivo.

我们和其他人已经观察到氧化剂是在 炎症组织损伤，并且已知是通过 刺激白细胞。 为了评估 氧化剂在炎症性疾病的致病性，它会 这对于确定氧化剂的细胞和分子靶点至关重要。 申请人建议研究特定氧化剂对 细胞的分子靶点。 特别是，拟议的研究旨在： 1.0 H202细胞靶点的定义 1.1 HOCl和氯胺的细胞靶点的定义 1.2来源于以下物质的氧化剂的影响的测定 刺激的中性粒细胞对靶细胞的作用，特别强调 DNA. 一旦在分子水平上确定了氧化剂的特定目标， 可以确定氧化剂参与， 炎症性疾病通过寻找这些形成 体内的"脚印"。