FUNCTIONS OF GLYCOPROTEIN D IN HERPESVIRUS INFECTION

糖蛋白 D 在疱疹病毒感染中的功能

• 批准号: 3455213
• 负责人: A. OVETA FULLER
• 金额: $ 10.69万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3455213
• 关键词: Alphaherpesvirinae; Herpes simplex disease; antiviral agents; cell cell interaction; cell fusion; chimeric proteins; electron microscopy; enzyme linked immunosorbent assay; flow cytometry; fluorescent dye /probe; gene expression; glycoproteins; immunocytochemistry; immunoprecipitation; membrane activity; membrane fusion; protein structure function; suid alphaherpesvirus 1; tissue /cell culture; virus infection mechanism; virus protein; virus replication

Glycoprotein D (gD) of herpes simplex virus (HSV) is required for successful infection. It is strongly implicated in virus penetration, in viral-induced cell-cell fusion, in exclusion of progeny virus and possibly, in stable attachment. However, the specific events that are mediated by gD and how it performs these functions is not yet known. The objective of this proposal is to determine the molecular basis of gD function for herpesviruses using biochemical, genetic, immunochemical and electron microscopy approaches. The specific aims are (a) to determine the step in HSV penetration that gD mediates that is hypothesized to differ form that mediated by other essential glycoproteins (gB and gH) (b) to determine other products besides gD that are required to mediate viral induced cell- cell fusion and (c) to determine functional similarity between gD and its homolog, gp50, in pseudorabies virus that it mediates a tighter adhesion between biological membranes. Evidence for or against this hypothesis will be provided by results from the proposed research. Understanding the molecular basis of gD function for HSV and PRV will provide information about critical events in herpesvirus entry and virus induced cell-cell fusion that will be relevant to other enveloped viruses which cause diseases. Since membrane fusion and membrane transit and frequent and essential biological events, this research will contribute to better understanding of this universal process.

单纯疱疹病毒(HSV)糖蛋白D(GD)是 成功感染。它与病毒的渗透密切相关，在 病毒诱导的细胞-细胞融合，排除了后代病毒，可能， 在稳定的依恋中。然而，由GD调解的具体事件 目前还不知道它是如何执行这些功能的。的目标是 这项建议是为了确定GD功能的分子基础 利用生化、遗传、免疫化学和电子技术检测疱疹病毒 显微镜走近了。具体目标是(A)确定 假设与GD介导的HSV穿透不同 由其他必需糖蛋白(Gb和Gh)(B)介导确定 除GD外，还需要其他介导病毒诱导细胞的产品- 细胞融合和(C)确定GD和ITS之间的功能相似性 伪狂犬病病毒中的同系物gp50，它介导更紧密的黏附 在生物膜之间。支持或反对这一假设的证据将 由拟议研究的结果提供。了解 HSV和PRV gD功能的分子基础将提供信息 关于疱疹病毒进入和病毒诱导的细胞-细胞的关键事件 与其他包膜病毒相关的融合，导致 疾病。由于膜融合和膜转运的频繁和 重要的生物事件，这项研究将有助于更好地 理解这一普遍过程。