FUNCTIONS OF GLYCOPROTEIN D IN HERPESVIRUS INFECTION

糖蛋白 D 在疱疹病毒感染中的功能

• 批准号: 3455214
• 负责人: A. OVETA FULLER
• 金额: $ 10.92万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3455214
• 关键词: Alphaherpesvirinae; Herpes simplex disease; antiviral agents; cell cell interaction; cell fusion; chimeric proteins; electron microscopy; enzyme linked immunosorbent assay; flow cytometry; fluorescent dye /probe; gene expression; glycoproteins; immunocytochemistry; immunoprecipitation; membrane activity; membrane fusion; protein structure function; suid alphaherpesvirus 1; tissue /cell culture; virus infection mechanism; virus protein; virus replication

Glycoprotein D (gD) of herpes simplex virus (HSV) is required for successful infection. It is strongly implicated in virus penetration, in viral-induced cell-cell fusion, in exclusion of progeny virus and possibly, in stable attachment. However, the specific events that are mediated by gD and how it performs these functions is not yet known. The objective of this proposal is to determine the molecular basis of gD function for herpesviruses using biochemical, genetic, immunochemical and electron microscopy approaches. The specific aims are (a) to determine the step in HSV penetration that gD mediates that is hypothesized to differ form that mediated by other essential glycoproteins (gB and gH) (b) to determine other products besides gD that are required to mediate viral induced cell- cell fusion and (c) to determine functional similarity between gD and its homolog, gp50, in pseudorabies virus that it mediates a tighter adhesion between biological membranes. Evidence for or against this hypothesis will be provided by results from the proposed research. Understanding the molecular basis of gD function for HSV and PRV will provide information about critical events in herpesvirus entry and virus induced cell-cell fusion that will be relevant to other enveloped viruses which cause diseases. Since membrane fusion and membrane transit and frequent and essential biological events, this research will contribute to better understanding of this universal process.

单纯疱疹病毒（HSV）的糖蛋白D（gD）是 成功感染。 它与病毒的侵入密切相关， 病毒诱导的细胞-细胞融合，排除子代病毒， 在稳定的连接中。 然而，由gD介导的特定事件 以及它如何执行这些功能还不知道。 的目标 这项建议是为了确定gD功能的分子基础， 利用生物化学、遗传学、免疫化学和电子技术 显微镜方法。 具体目标是：（a）确定 假设gD介导的HSV渗透不同于 由其他必需糖蛋白（gB和gH）（B）介导，以确定 除了gD之外，还需要其他产物来介导病毒诱导的细胞- 细胞融合和（c）确定gD及其之间的功能相似性 在伪狂犬病病毒中的同源物gp 50，它介导更紧密的粘附 在生物膜之间。 支持或反对这一假设的证据将 由拟议研究的结果提供。 了解 HSV和PRV gD功能的分子基础将提供信息 关于疱疹病毒进入和病毒诱导的细胞-细胞中的关键事件 融合，将相关的其他包膜病毒，造成 疾病 由于膜融合和膜转运频繁， 重要的生物事件，这项研究将有助于更好地 理解这个普遍的过程。