TISSUE-SPECIFIC CONTROL OF MUSCLE GENE EXPRESSION
肌肉基因表达的组织特异性控制
基本信息
- 批准号:3457023
- 负责人:
- 金额:$ 4.43万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1988
- 资助国家:美国
- 起止时间:1988-04-01 至 1990-09-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Skeletal muscle differentiation is accompanied by the coordinate
induction of sarcomeric proteins. The appearance of these
proteins is preceded by a rise in the abundance of the
corresponding mRNAs reflecting an increase in transcriptional
activity. This transcriptional increase, in turn, is dependent upon
the presence of the correct cis- and trans- acting elements. We
have recently identified a nuclear factor in skeletal muscle which
binds specifically and with high affinity to an upstream sequence
element located 5' to the genes encoding the sarcomeric proteins,
a-actin and myosin light chain 2. This finding lends support to the
hypothesis that the sarcomeric proteins are coordinately induced
through the action of a limited number of trans-acting factors. In
the present application, we propose to extend these observations
by (i) Delineating the role of the upstream muscle specific,
sequence element in the regulation muscle gene expression (ii)
Purifying and characterizing the skeletal muscle nuclear factor(s)
that bind to this sequence (iii) Developing an in vitro transcription
system with which to rigorously assess the regulatory function of
muscle specific factors. Our goal, the purification of trans-acting
factors and the elucidation of the role of DNA binding sequences
in tissue-specific gene regulation, is the first step in
understanding the developmental cascade which eventually results
in myoblast differentiation.
骨骼肌的分化伴随着骨骼肌的
肌节蛋白的诱导。 的外观
蛋白质之前,
相应的mRNA反映了转录水平的增加,
活动 反过来,这种转录增加依赖于
存在正确的顺式和反式作用元件。 我们
最近在骨骼肌中发现了一种核因子,
以高亲和力特异性结合上游序列
位于编码肌节蛋白的基因的5 '端的元件,
α-肌动蛋白和肌球蛋白轻链2。 这一发现支持了
肌节蛋白协同诱导的假说
通过有限数量的反式作用因子的作用。 在
在本申请中,我们建议扩展这些观察
通过(i)描述上游肌肉特异性的作用,
调控肌肉基因表达的序列元件(ii)
骨骼肌核因子的纯化和表征
(iii)开发体外转录
严格评估监管职能的制度
肌肉特异性因素 我们的目标,反式作用的纯化
因素和DNA结合序列的作用的阐明
在组织特异性基因调控中,
理解最终导致
在成肌细胞分化中。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Rebecca L. Aft其他文献
Micrometastatic Disease and Isolated Tumor Cells as a Predictor for Additional Breast Cancer Axillary Metastatic Burden
- DOI:
10.1245/s10434-010-1255-1 - 发表时间:
2010-09-19 - 期刊:
- 影响因子:3.500
- 作者:
Amy Cyr;William E. Gillanders;Rebecca L. Aft;Timothy J. Eberlein;Feng Gao;Julie A. Margenthaler - 通讯作者:
Julie A. Margenthaler
Predictors of Primary Breast Abscesses and Recurrence
- DOI:
10.1007/s00268-009-0170-8 - 发表时间:
2009-08-08 - 期刊:
- 影响因子:2.500
- 作者:
Ankit Bharat;Feng Gao;Rebecca L. Aft;William E. Gillanders;Timothy J. Eberlein;Julie A. Margenthaler - 通讯作者:
Julie A. Margenthaler
Disparities related to socioeconomic status and access to medical care remain in the United States among women who never had a mammogram
- DOI:
10.1023/a:1024941626748 - 发表时间:
2003-06-01 - 期刊:
- 影响因子:2.100
- 作者:
Mario Schootman;Donna B. Jeffe;Anat H. Reschke;Rebecca L. Aft - 通讯作者:
Rebecca L. Aft
Rebecca L. Aft的其他文献
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{{ truncateString('Rebecca L. Aft', 18)}}的其他基金
Synergized Immune and Tumor Cell Bone Marrow Biomarkers to Predict Recurrence in Triple Negative Breast Cancer
协同免疫和肿瘤细胞骨髓生物标志物可预测三阴性乳腺癌的复发
- 批准号:
10491904 - 财政年份:2021
- 资助金额:
$ 4.43万 - 项目类别:
Synergized Immune and Tumor Cell Bone Marrow Biomarkers to Predict Recurrence in Triple Negative Breast Cancer
协同免疫和肿瘤细胞骨髓生物标志物可预测三阴性乳腺癌的复发
- 批准号:
10279058 - 财政年份:2021
- 资助金额:
$ 4.43万 - 项目类别:
ANALYSIS AND THERAPEUTIC TARGETING OF BREAST CANCER DISSEMINATED TUMOR CELLS
乳腺癌播散性肿瘤细胞的分析和治疗靶向
- 批准号:
9113483 - 财政年份:2013
- 资助金额:
$ 4.43万 - 项目类别:
ANALYSIS AND THERAPEUTIC TARGETING OF BREAST CANCER DISSEMINATED TUMOR CELLS
乳腺癌播散性肿瘤细胞的分析和治疗靶向
- 批准号:
8579477 - 财政年份:2013
- 资助金额:
$ 4.43万 - 项目类别:
ANALYSIS AND THERAPEUTIC TARGETING OF BREAST CANCER DISSEMINATED TUMOR CELLS
乳腺癌播散性肿瘤细胞的分析和治疗靶向
- 批准号:
8737811 - 财政年份:2013
- 资助金额:
$ 4.43万 - 项目类别:
ANALYSIS AND THERAPEUTIC TARGETING OF BREAST CANCER DISSEMINATED TUMOR CELLS
乳腺癌播散性肿瘤细胞的分析和治疗靶向
- 批准号:
8889642 - 财政年份:2013
- 资助金额:
$ 4.43万 - 项目类别:
ANALYSIS AND THERAPEUTIC TARGETING OF BREAST CANCER DISSEMINATED TUMOR CELLS
乳腺癌播散性肿瘤细胞的分析和治疗靶向
- 批准号:
9318459 - 财政年份:2013
- 资助金额:
$ 4.43万 - 项目类别:
Prognostic Marker Analysis of Disseminated Cancer Cells
播散性癌细胞的预后标志物分析
- 批准号:
6968924 - 财政年份:2005
- 资助金额:
$ 4.43万 - 项目类别:
Prognostic Marker Analysis of Disseminated Cancer Cells
播散性癌细胞的预后标志物分析
- 批准号:
7140124 - 财政年份:2005
- 资助金额:
$ 4.43万 - 项目类别:
TISSUE-SPECIFIC CONTROL OF MUSCLE GENE EXPRESSION
肌肉基因表达的组织特异性控制
- 批准号:
3457022 - 财政年份:1988
- 资助金额:
$ 4.43万 - 项目类别:
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