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ANALYSIS OF TARGET GENES ACTIVATED--JUN ONCOPROTEIN

激活靶基因分析--JUN ONCOPROTEIN

基本信息

批准号：
2094497
负责人：
TIMOTHY J BOS
金额：
$ 9.11万
依托单位：
EASTERN VIRGINIA MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-08-01 至 1995-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2094497
关键词：
cell growth regulation cytogenetics gene conversion gene expression gene mutation genetic library genetic regulatory element genetic transcription genetic translation laboratory rabbit microorganism genetics nucleic acid sequence oncogenes structural genes transcription factor

项目摘要

The mechanisms governing the regulation of cell growth are fundamental to the understanding of tumorigenesis. These mechanisms include signal chains that can be affected by oncogenes. In order to analyze these mechanisms, one must know normal and aberrant functions of oncogenes, their targets and effects on cellular signalling. The long term objective of this work is to gain insight into the mechanisms by which oncogenes turn normal cells into cancer cells. The specific aims focus on the identification and molecular characterization of oncogenically important target genes activated by the jun oncoprotein. This knowledge will be invaluable in understanding the process by which oncogenes induce cell transformation as well as the steps involved in normal growth control. This study will be divided into three parts. The first involves the identification of primary target genes activated by jun. This will be done by subtraction screening of a cDNA library generated immediately after induction of expression of v-jun, against an uninduced library. The second area involves the determination of oncogenic potential of each target identified. This will be performed by assaying for cell transformation in the potential large number of targets, these assays will be performed with pools of DNA. Important targets from transformation positive pools can then be sorted out. The third area deals with a molecular analysis of oncogenically important target gene(s) and a structural and functional characterization of their products.

控制细胞生长调节的机制对于对肿瘤发生的理解。这些机制包括信号链会受到致癌基因的影响。为了分析这些机制，人们必须了解癌基因的正常和异常功能，它们的靶点，对细胞信号的影响。这项工作的长期目标是深入了解致癌基因将正常细胞转化为癌细胞具体目标集中在鉴定和分子表征致癌重要的靶基因激活的 jun癌蛋白。这些知识对于理解癌基因诱导细胞转化的过程以及参与正常生长控制。本研究将分为三个部分。第一个涉及识别jun激活的主要靶基因。这将在通过差减筛选后立即产生的cDNA文库针对未诱导文库诱导v-jun的表达。第二区域涉及确定每个靶点的致癌潜力鉴定这将通过测定细胞转化来进行，由于潜在的大量靶标，这些试验将使用 DNA池转化阳性样本池中的重要靶标可以解决问题第三个领域涉及的分子分析，致癌重要的靶基因和结构和功能他们的产品特征。