OTITIS MEDICA--IN VIVO MODEL

中耳炎（OM）是最常见的儿科疾病， 医生的办公室访问。 使用动物模型研究OM 有很多的知识，有很多的知识， 发病机制和治疗方法已经积累。 现款车型 利用生物化学、细菌学和组织学分析， 测量宿主的炎症反应。 这些模型 无论是在体外还是离体， 实时生理变化。 最近，使用磁共振成像的oM体内模型 (MRI)描述了通过造影剂增强的图像。 这种技术 能够分辨诸如中耳（ME）之类的薄结构 通过组织学比较验证的粘膜。 此外，急性 慢性OM可根据特征性 造影剂的洗脱率。 这种方法将提供独特的机会，调查 体内ME粘膜，因为它与血管通透性有关。 这 模型允许纵向数据采集， 而不是过去事件的累积。 这项调查将使用龙猫OM模型来完善 成像方法的灵敏度和特异性。 的 急性和慢性炎症对洗脱率的影响 将记录造影剂。 此外，影响 将评估主要药物组对炎症过程的影响 使用介入模型。 MRI技术的结果将 与其他血管渗透性方法相关， 将该方法扩展到其他物种，如家猫 将被启动。 尽管这种增强的成像方法不是 虽然还没有被批准用于人类，但进一步的研究可能会导致未来 应用这项技术。

Otitis media (OM) is the most common pediatric illness requiring physicians' office visits. The use of animal models to study OM is well established and a large amount of knowledge on its pathogenesis and treatment has been amassed. Current models utilize biochemical, bacteriologic, and histologic assays to measure the inflammatory response of the host. These models are either in vitro or ex vivo and lack the ability to assess dynamic real-time physiologic changes. Recently, an in vivo model of oM using magnetic resonance imaging (MRI) enhanced by a contrast agent was described. This technique is capable of resolving thin structures such as the middle-ear (ME) mucosa as validated by a histologic comparison. In addition, acute and chronic OM can be differentiated based on characteristic washout rates of the contrast agent. This methodology would offer unique opportunity to investigate the ME mucosa in vivo as it relates to vascular permeability. This model allows longitudinal data acquisition which represents current events rather than cumulation of past events. This investigation will use the chinchilla OM models to refine the sensitivity and specificity of the imaging methodology. The effects of acute and chronic inflammation on the washout rate of the contrast agent will be documented. Additionally, the effects of major drug groups on the inflammatory process will be assessed using interventional models. Results from the MRI technique will be correlated to other methods of vascular permeability and extension of the method to other species such as the domestic cat will be initiated. Although this enhanced imaging method is not yet approved for humans, further investigation may result in future application of this technique.