OTITIS MEDICA--IN VIVO MODEL

中耳炎(OM)是最常见的儿科疾病 医生的办公室来访。利用动物模型研究OM 已经很成熟了，并且有大量关于它的知识 发病机制和治疗已有一定的积累。当前型号 利用生化、细菌学和组织学分析 测量宿主的炎症反应。这些型号是 无论是在体外还是体外，都缺乏评估动态变化的能力 实时生理变化。 最近，一种利用磁共振成像的OM活体模型 本文描述了一种造影剂增强的磁共振成像。这项技术 能够解析薄结构，如中耳(ME) 通过组织学比较证实的粘膜。此外，急性 根据特征可以区分慢性OM 造影剂的洗脱率。 这种方法将提供独特的机会来调查 肌动蛋白与血管通透性的关系。这 模型允许进行代表电流的纵向数据采集 事件，而不是过去事件的累积。 这项调查将使用栗鼠OM模型来提炼 影像方法学的敏感性和特异性。这个 急、慢性炎症对牙周炎冲洗率的影响 造影剂将被记录在案。此外，它的影响 将对炎症过程中的主要药物组进行评估 使用干预性模型。核磁共振技术的结果将 与血管通透性的其他方法和 将该方法推广到其他物种，如家猫 将会被启动。尽管这种增强的成像方法不是 尽管已被批准用于人类，但未来可能会有进一步的研究 这项技术的应用。

Otitis media (OM) is the most common pediatric illness requiring physicians' office visits. The use of animal models to study OM is well established and a large amount of knowledge on its pathogenesis and treatment has been amassed. Current models utilize biochemical, bacteriologic, and histologic assays to measure the inflammatory response of the host. These models are either in vitro or ex vivo and lack the ability to assess dynamic real-time physiologic changes. Recently, an in vivo model of oM using magnetic resonance imaging (MRI) enhanced by a contrast agent was described. This technique is capable of resolving thin structures such as the middle-ear (ME) mucosa as validated by a histologic comparison. In addition, acute and chronic OM can be differentiated based on characteristic washout rates of the contrast agent. This methodology would offer unique opportunity to investigate the ME mucosa in vivo as it relates to vascular permeability. This model allows longitudinal data acquisition which represents current events rather than cumulation of past events. This investigation will use the chinchilla OM models to refine the sensitivity and specificity of the imaging methodology. The effects of acute and chronic inflammation on the washout rate of the contrast agent will be documented. Additionally, the effects of major drug groups on the inflammatory process will be assessed using interventional models. Results from the MRI technique will be correlated to other methods of vascular permeability and extension of the method to other species such as the domestic cat will be initiated. Although this enhanced imaging method is not yet approved for humans, further investigation may result in future application of this technique.