GROWTH-INHIBITORY FACTOR ACTION ON GH4 PITUITARY CELLS

描述(改编自申请人的摘要)：本报告的目标 应用是利用GH4细胞系来阐明细胞 生长因子抑制脑垂体细胞增殖的过程。它 被提议研究转化生长因子-α和转化生长因子-β的几个方面 生长抑制机制。首先，将检查转化生长因子-α是否 或者，转化生长因子-β阻断了细胞周期的特定状态。那它就会是 检测转化生长因子-α或转化生长因子-β是否通过 防止明确的增长刺激因素的作用，提升 细胞内游离钙，或增强细胞与基质的粘附力。

DESCRIPTION (adapted from applicant's abstract): The goal of this application is to utilize the GH4 cell line to elucidate the cellular processes by which growth factors inhibit pituitary cell proliferation. It is proposed to investigate several aspects of TGF-alpha and TGF-beta growth-inhibitory mechanisms. First, it will be examined whether TGF-alpha or TGF-beta blocks specific states of the cell cycle. Then it will be examined whether TGF-alpha or TGF-beta inhibits cell proliferation by preventing the actions of defined growth-stimulatory factors, elevating free cytosolic calcium, or enhancing cell-substratum adhesion.