PROTEIN DYNAMICS IN BACTERIORHODOPSIN & RHODOPSIN

细菌视紫红质的蛋白质动力学

• 批准号: 3467063
• 负责人: Carey K Johnson
• 金额: $ 9.12万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3467063
• 关键词: Halobacteriaceae; Raman spectrometry; bacteriorhodopsins; chemical binding; conformation; cow; fluorescence polarization; laser spectrometry; molecular dynamics; rhodopsin; solvents; temperature; thermodynamics; tyrosine; ultraviolet spectrometry

The aim of these experiments is to detect and measure internal motion in bacteriorhodopsin with 1 ps to 40 ps (initially) resolution over time regimes from 1 ps to 1 ms, as a probe of protein dynamics and protein- chromophore interaction. Experiments are proposed to study: 1) the amplitude and rate of overall motion of the bacteriorhodopsin chromophore in its light-adapted state and in intermediates J, K, and L; 2) the relaxation of strained conformations of the chromophore; 3) activation barriers to chromophore motion; 4) the temperature and solvent dependence of the binding site geometry; 5) the solvent viscosity dependence of chromophore relaxation; 6) proton coordination to a tyrosinate adjacent to the chromophore; and 7) activation volumes for chromophore evolution. Experiments to study low-temperature dynamics and room-temperature picosecond conformational evolution are also proposed for rhodopsin. Unique aspects of our approach are: 1) the accessibility of time regimes from 1 ps to 1 ms on a single instrument; 2) detection of internal motion by a probe of anisotropy decay that is not limited by the excited-state lifetime; 3) detailed measurement of relaxation rates of specific vibrational features; 4) accurate studies of the temperature and pressure dependence of relaxation rates. The approach proposed is to use a natural chromophore as a probe of protein dynamics. This problem is attacked by several picosecond laser techniques capable of generating specific and detailed information about structure and dynamics.

这些实验的目的是检测和测量内部运动