LASER ANGIOPLASTY GUIDANCE BY FIBEROPTIC FLUORIMETRY

通过光纤荧光测定法指导激光血管成形术

• 批准号: 3471284
• 负责人: LAWRENCE I DECKELBAUM
• 金额: $ 7.9万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3471284
• 关键词: arteriosclerosis; artery occlusion; blood; clinical biomedical equipment; coronary artery; fiber optics; fluorescence spectrometry; fluosol; heart revascularization; online computer; saline; tetracyclines

Currently the major impediment to safe and effective laser angioplasty is the high incidence of arterial perforation. The aim of this research project is to evaluate the hypothesis that fiberoptic fluorimetry can distinguish atherosclerotic from normal artery and thereby guide the delivery of laser energy for plaque ablation. Preliminary investigation has shown that characteristic fluorescence can be induced in human atheromas by low power laser radiation. A fiberoptic catheter could there transmit low power radiation forfluorescence analysis and if correctly targeted, subsequently transmit high power laser radiation for plaque ablation. This proposal will evaluate fiberoptic fluorimetry of normal and atherosclerotic human cadaveric aorta, iliofemoral and coronary arteries using a helium cadmium laser (wavelength 325 nm) for fluorescence induction, and a microcomputer based optical multichannel analyzer for fluorescence analysis. The specific aims are to: 1) derive a discriminant function to differentiate atherosclerotic from normal arterial tissue histology based on laser-induced fluorescence characteristics; 2) evaluate fiberoptic laser-induce fluorescence spectroscopy in a fluid environment (saline, blood, or Fluosol); 3) evaluate the arterial fluorescence changes as plaque ablation proceeds using fiberoptic fluorimetry in conjunction with high peak power pulsed Alexandrite laser ablation (wavelength 378 nm frequency doubled); 4) evaluate whether exogenously administered fluorescent compounds (tetracycline hematoporphyrin derivative, or histologic dyes) localize to atherosclerotic plaque and enhance the diagnostic capabilities of fiberoptic fluorimetry; 5) evaluate the ability of computerized fiberoptic fluorimetry guidance to achieve selective plaque ablation without damage to the underlying arterial wall in an in vitro laser angioplasty model. It is hoped that the currently high risk of arterial perforation during laser angioplasty can be eliminated by fiberoptic fluorimetry guidance. In addition, fluorimetry guided laser ablation may have broad application in other areas of medicine by the potential to achieve selective tissue ablation of any mass that is distinguishable by fluorescence properties.

目前安全有效激光的主要障碍 血管成形术中动脉穿孔的发生率很高。 目的 该研究项目的目的是评估以下假设 光纤荧光测定法可以区分动脉粥样硬化和正常动脉粥样硬化 动脉，从而引导激光能量输送至斑块 消融。 初步调查显示，具有以下特点： 低功率可在人体动脉粥样化中诱导荧光 激光辐射。 光纤导管可以在那里传输低 用于荧光分析的功率辐射，如果正确的话 瞄准，随后发射高功率激光辐射 斑块消融。 该提案将评估光纤 正常和动脉粥样硬化人类尸体主动脉的荧光测定， 使用氦镉激光检查髂股动脉和冠状动脉 （波长 325 nm）用于荧光诱导，以及 基于微机的光学多道分析仪 荧光分析。 具体目标是： 1）推导出判别函数来区分 动脉粥样硬化来源于正常动脉组织的组织学基础 激光诱导荧光特性的研究； 2) 评估光纤激光诱导荧光光谱 在流体环境中（盐水、血液或 Fluosol）； 3)评估动脉荧光变化作为斑块 结合使用光纤荧光测定法进行消融 采用高峰值功率脉冲紫翠玉激光烧蚀 （波长378纳米频率加倍）； 4) 评估是否外源给予荧光 化合物（四环素血卟啉衍生物，或 组织学染料）定位于动脉粥样硬化斑块和 增强光纤诊断能力 荧光测定法； 5) 评估计算机化光纤荧光测定的能力 指导实现选择性斑块消融，无需 体外激光对下层动脉壁的损伤 血管成形术模型。 希望目前动脉穿孔的高风险 激光血管成形术期间可以通过光纤消除 荧光测定指导。 此外，荧光引导激光 消融可能在其他医学领域具有广泛的应用 实现对任何肿块进行选择性组织消融的潜力 可以通过荧光特性来区分。