LASER ANGIOPLASTY GUIDANCE BY FIBEROPTIC FLUORIMETRY

通过光纤荧光测定法指导激光血管成形术

• 批准号: 3471282
• 负责人: LAWRENCE I DECKELBAUM
• 金额: $ 12.84万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3471282
• 关键词: arteriosclerosis; artery occlusion; blood; clinical biomedical equipment; coronary artery; fiber optics; fluorescence spectrometry; heart revascularization; online computer; saline; tetracyclines

Currently the major impediment to safe and effective laser angioplasty is the high incidence of arterial perforation. The aim of this research project is to evaluate the hypothesis that fiberoptic fluorimetry can distinguish atherosclerotic from normal artery and thereby guide the delivery of laser energy for plaque ablation. Preliminary investigation has shown that characteristic fluorescence can be induced in human atheromas by low power laser radiation. A fiberoptic catheter could there transmit low power radiation forfluorescence analysis and if correctly targeted, subsequently transmit high power laser radiation for plaque ablation. This proposal will evaluate fiberoptic fluorimetry of normal and atherosclerotic human cadaveric aorta, iliofemoral and coronary arteries using a helium cadmium laser (wavelength 325 nm) for fluorescence induction, and a microcomputer based optical multichannel analyzer for fluorescence analysis. The specific aims are to: 1) derive a discriminant function to differentiate atherosclerotic from normal arterial tissue histology based on laser-induced fluorescence characteristics; 2) evaluate fiberoptic laser-induce fluorescence spectroscopy in a fluid environment (saline, blood, or Fluosol); 3) evaluate the arterial fluorescence changes as plaque ablation proceeds using fiberoptic fluorimetry in conjunction with high peak power pulsed Alexandrite laser ablation (wavelength 378 nm frequency doubled); 4) evaluate whether exogenously administered fluorescent compounds (tetracycline hematoporphyrin derivative, or histologic dyes) localize to atherosclerotic plaque and enhance the diagnostic capabilities of fiberoptic fluorimetry; 5) evaluate the ability of computerized fiberoptic fluorimetry guidance to achieve selective plaque ablation without damage to the underlying arterial wall in an in vitro laser angioplasty model. It is hoped that the currently high risk of arterial perforation during laser angioplasty can be eliminated by fiberoptic fluorimetry guidance. In addition, fluorimetry guided laser ablation may have broad application in other areas of medicine by the potential to achieve selective tissue ablation of any mass that is distinguishable by fluorescence properties.

目前，安全有效激光的主要障碍是 血管成形术是动脉穿孔的高发生率。 目的 这个研究项目的目的是评估假设， 光纤荧光法可以区分动脉粥样硬化和正常 从而引导用于斑块的激光能量的输送 消融术 初步调查显示， 荧光可以通过低功率在人动脉粥样硬化中诱导 激光辐射 光纤导管可以在那里传输低 用于荧光分析的功率辐射，如果正确 有针对性的，随后发射高功率激光辐射， 斑块消融 该提案将评估光纤 正常和动脉粥样硬化的人尸体主动脉的荧光测定， 髂股动脉和冠状动脉使用氦镉激光 （波长325 nm）用于荧光诱导，和 微电脑光学多道分析仪 荧光分析 具体目标是： 1)推导出判别函数， 动脉粥样硬化从正常动脉组织的组织学基础 激光诱导荧光特性; 2)评价光纤激光诱导荧光光谱 在液体环境中（生理盐水、血液或Fluosol）; 3)评价动脉荧光变化作为斑块 使用光纤荧光测定法结合 用高峰功率脉冲紫翠宝石激光烧蚀 （波长378 nm倍频）; 4)评价外源性给予荧光 化合物（四环素血卟啉衍生物，或 组织学染料）定位于动脉粥样硬化斑块， 增强光纤的诊断能力 荧光测定法; 5)评价计算机化光纤荧光分析的能力 指导实现选择性斑块消融， 体外激光对动脉壁的损伤 血管成形术模型 希望目前动脉穿孔的高风险 在激光血管成形术中， 荧光测定法指导。 此外，荧光法引导的激光 消融可以广泛应用于其它医学领域， 实现任何肿块的选择性组织消融的可能性， 可通过荧光特性区分。