RAMAN STUDIES OF SULFITE REDUCTASES AND OTHER ENZYMES

亚硫酸盐还原酶和其他酶的拉曼研究

• 批准号: 3466307
• 负责人: KWOK T YUE
• 金额: $ 8.42万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3466307
• 关键词: Bacillaceae; Desulfovibrio; Raman spectrometry; chemical binding; chromophore; enzyme structure; enzyme substrate; ligands; metalloenzyme; metalloproteins; oxidation reduction reaction; sulfite reductase

We propose to study how protein environment and/or chromophore interaction controls the properties and reactivity of a chromophore using resonance Raman spectroscopy by examining siroheme and iron-sulfur clusters in different sulfite reductases from sulfate reducing bacteria. Resonance Raman spectroscopy can yield detailed molecular information of the chromophore in situ unavailable by other methods. Sulfite reductases catalyze the 6-electron reduction of sulfite to sulfide. The reduction is crucial for synthesis of sulfur-containing cell constituents and in terminal respiration for sulfate reducing bacteria. Although sulfite reductases contain the same two chromophores, their properties are quite different, making them ideal systems for studying structural changes with reactivity. In order to understand the Raman spectra of the chromophores, we plan to study the chromophores and their analogs in various solutions and environments. We also propose to study the mechanism of sulfite reductases by examining changes in the chromophores during catalysis, at different redox potentials, and bound to different substrates. We also propose to characterize the chromophores of other iron- sulfur proteins from sulfate reducing bacteria.

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