MOLECULAR ANALYSIS OF SEXUAL DEVELOPMENT IN NEUROSPORA

神经孢子虫性发育的分子分析

• 批准号: 3468774
• 负责人: MARY A NELSON
• 金额: $ 9.4万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3468774
• 关键词: DNA footprinting; Neurospora; biochemical evolution; confocal scanning microscopy; fungal genetics; gene expression; gene interaction; gene mutation; genetic mapping; genetic regulatory element; genetic transcription; molecular cloning; northern blottings; nucleic acid sequence; phenotype; protein structure function; sex differentiation; southern blotting

The long term objective of this research is to characterize the sexual development of the filamentous fungi at the molecular level. Although these organisms are of vast economic and social importance, very little is known of their development. The much-studied bread mold Neurospora crassa will be used as the model organism. Neurospora undergoes a complex process of sexual differentiation; a number of the regulatory and target (mating-specific) genes that are involved in this process have already been identified. The structures and functions of these genes will be examined, as will the control of their expression and the interactions between their gene products. The roles of the mating-specific genes in sexual development will be determined using a reverse-genetic approach, and the phenotypes observed when these genes are dysfunctional will be characterized microscopically. The evolutionary conservation of the mating-specific genes will be examined. The factors that cause these genes to be expressed specifically during sexual development will be studied; in preliminary experiments, three control pathways were identified. The interactions between these control pathways will be examined, and a search for other elements involved in the control of gene expression during sexual development will be executed. The structures (nucleotide sequences) of a regular gene (fmf-1) and selected mating-specific target genes will be determined, and the properties of their encoded products examined. An in vitro analysis of the regulatory elements recognized during sexual development will be carried out using mobility shift assays and DNA footprinting experiments; this analysis will serve to identify important control elements and further define the roles played by the regulatory gene products. Finally, the hierarchy of events required for normal sexual development in Neurospora will be examined, by determining the effects of selected processes on the overall sexual developmental pathway. Some filamentous fungi are exploited for the production of useful compounds, but others cause devastating diseases. Basic information about the control of their life processes will allow judicious use of the beneficial fungi, and will also further efforts to control the pathogens.

这项研究的长期目标是描述 丝状真菌在分子水平上的发育。虽然 这些生物体具有巨大的经济和社会重要性，几乎没有 都知道它们的发展。被广泛研究的面包霉菌--脉孢霉 将以克拉沙菌作为模式生物。脉孢子虫经历了一个 复杂的性别分化过程；许多调节和 参与这一过程的靶(交配特异性)基因有 已经被确认了。这些基因的结构和功能 将被审查，以及对其表达的控制和 它们的基因产物之间的相互作用。 交配特定基因在性发育中的作用将是 使用反向遗传方法确定的，以及观察到的表型 当这些基因功能失调时，将在显微镜下对其进行表征。 交配特异基因的进化保守性将是 检查过了。导致这些基因表达的因素 特别是在性发育过程中进行研究；在初步 实验中，确定了三条调控途径。互动 将检查这些控制路径之间的关系，并搜索其他 参与有性繁殖过程中基因表达调控的因素 将执行开发。A的结构(核苷酸序列) 常规基因(FMF-1)和选定的交配特异靶基因将是 确定，并检查其编码产品的属性。An In 性交过程中识别的调节元件的体外分析 将使用迁移率变化分析和DNA进行开发 足迹实验；这一分析将有助于识别重要的 控制要素，并进一步定义监管机构所扮演的角色 基因产品。最后，正常所需的事件层级 将检查脉孢子菌的性发育，通过确定 选择的过程对全局性发育的影响 路径。 一些丝状真菌被开发用于生产有用的 化合物，但其他化合物会导致毁灭性的疾病。基本信息 关于他们生命过程的控制将允许明智地使用 有益的真菌，也将进一步努力控制病原体。