RETROPOSON MAP OF THE HUMAN GENOME
人类基因组逆算子图谱
基本信息
- 批准号:3470812
- 负责人:
- 金额:$ 11.48万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1990
- 资助国家:美国
- 起止时间:1990-06-01 至 1995-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The ultimate goal of this research is to create a retroposon map of the
human genome. Mapping will be approached using techniques of high
resolution in situ hybridization and quantitative image analysis, combined
with Southern blot analysis of somatic cell hybrids containing single human
chromosomes or chromosome bands. There are two major retroposon families
and 6 minor families in humans accounting for 15-20% of the mass of the
genome and occurring in 1,000-1,000,000 copies per haploid genome.
Although these elements are actively shaping the genome and are known to be
associated with insertional and deletional mutations in humans, the process
of retroposition, and the functions of the progenitor sequences and of
their descendants are unknown.
We have recently confirmed that the two major retroposons, Alu and L1 are
not randomly distributed throughout the genome but instead are clustered in
regions defined by the human metaphase chromosome bands, the domains
related to DNA replication, prophase condensation, crossing over, gene
density, and fragile sites.
The aims of this proposal are:
1. To determine the retroposon map of human chromosomes using high
resolution in situ hybridization and computer aided image analysis.
Biotinylated probes for 8 families of human retroposons will be
investigated, including Alu, MstII, SINE-R, O-LTR, L1 and its subfragments,
L2HS, THE-1 and HSRTVL-H.
2. To create a retroposon map of single human chromosomes 17 and 21 and
their chromosome bands using Southern blot hybridization of the above
retroposon families to somatic cell hybrids carrying single human
chromosomes or bands. A panel of restriction enzymes will be used to
screen each chromosome for distinct retroposon subfamilies.
3. To create a retroposon map of the human X chromosome, and to identify
and clone a subfamily of putatively full length L1 retroposons visible on
Southern blots as a single band and concentrated in the centromeric region
bordering the region of the putative X inactivation center. Using this
specific subfamily of retroposons of L1 as markers for the region, the
neighboring non L1 sequences will be identified to help elucidate the
molecular structure of the region.
These studies should provide further molecular information on the basis of
chromosome organization and may help close the gap in genome analysis to
allow the identification of new DNA sequences in defined chromosomal
regions, and the detection and cloning of some chromosomal changes below
the limit of cytogenetic analysis.
这项研究的最终目标是建立一个逆转录酶图谱,
人类基因组 映射将采用高技术,
分辨率原位杂交和定量图像分析,结合
用Southern印迹分析含有单个人的体细胞杂种
染色体或染色体带。 有两个主要的逆转录酶家族
人类有6个小家族,占人类总人口的15-20%。
每个单倍体基因组有1000 - 1000000个拷贝。
尽管这些元素正在积极地塑造基因组,
与人类的插入和缺失突变相关,
以及前体序列的功能,
他们的后代是未知的。
我们最近已经证实,两个主要的逆转录酶,Alu和L1,
不是随机分布在整个基因组中,而是聚集在
由人类中期染色体带定义的区域,
与DNA复制、前期凝聚、交换、基因
密度和脆弱的网站。
这项建议的目的是:
1. 用高效液相色谱法测定人类染色体的逆转录酶图谱,
分辨率原位杂交和计算机辅助图像分析。
8个人类逆转录子家族的生物素化探针将被
包括Alu、MstII、SINE-R、O-LTR、L1及其亚片段,
L2HS、THE-1和HSRTVL-H。
2. 创建一个人类17号和21号染色体的逆转录酶图谱,
用Southern印迹杂交的方法,
携带单个人类基因的体细胞杂种
染色体或带。 一组限制性内切酶将用于
在每条染色体上筛选不同的逆转录酶亚家族。
3. 创建人类X染色体的逆转录酶图谱,
并克隆一个puppatient全长L1逆转录子亚家族,
Southern杂交显示为单一条带,且集中在着丝粒区
与假定的X染色体失活中心的区域接壤。 使用此
作为该区域标记的L1反转录子的特定亚家族,
相邻的非L1序列将被识别,以帮助阐明
区域的分子结构。
这些研究应该提供进一步的分子信息的基础上,
染色体组织,并可能有助于缩小基因组分析中的差距,
允许在确定的染色体中鉴定新的DNA序列,
区域,并检测和克隆下面的一些染色体变化
细胞遗传学分析的局限性
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JULIE RUTH KORENBERG其他文献
JULIE RUTH KORENBERG的其他文献
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{{ truncateString('JULIE RUTH KORENBERG', 18)}}的其他基金
A Computational Framework for Mapping Long Range Genetic Circuits
绘制长距离遗传电路的计算框架
- 批准号:
7845097 - 财政年份:2009
- 资助金额:
$ 11.48万 - 项目类别:
A Computational Framework for Mapping Long Range Genetic Circuits
绘制长距离遗传电路的计算框架
- 批准号:
7938599 - 财政年份:2009
- 资助金额:
$ 11.48万 - 项目类别:
Williams Syndrome: The Molecular Genetic Characterization
威廉姆斯综合症:分子遗传特征
- 批准号:
7003873 - 财政年份:2004
- 资助金额:
$ 11.48万 - 项目类别:
Down syndrome: Bridging Genes and Neural Pathways
唐氏综合症:连接基因和神经通路
- 批准号:
7177523 - 财政年份:2003
- 资助金额:
$ 11.48万 - 项目类别:
Down syndrome: Bridging Genes and Neural Pathways
唐氏综合症:连接基因和神经通路
- 批准号:
7018513 - 财政年份:2003
- 资助金额:
$ 11.48万 - 项目类别:
Down syndrome: Bridging Genes and Neural Pathways
唐氏综合症:连接基因和神经通路
- 批准号:
6832836 - 财政年份:2003
- 资助金额:
$ 11.48万 - 项目类别:
Down syndrome: Bridging Genes and Neural Pathways
唐氏综合症:连接基因和神经通路
- 批准号:
6700035 - 财政年份:2003
- 资助金额:
$ 11.48万 - 项目类别:
Down syndrome: Bridging Genes and Neural Pathways
唐氏综合症:连接基因和神经通路
- 批准号:
6760096 - 财政年份:2003
- 资助金额:
$ 11.48万 - 项目类别: