CELLULAR MECHANISMS IN UTERINE PROSTAGLANDIN SYNTHESIS

子宫腺素（PGs）在着床中起重要作用， 分娩、黄体溶解和母亲对妊娠的识别。然而 调节子宫PG合成的因素知之甚少。 的 本提案所述实验的目的是 表征调节这种合成的细胞内机制。 催产素刺激子宫组织合成前列腺素。 的 所描述的实验具有两个特定目的。 一是考察 磷脂酶C在介导神经元兴奋效应中的作用 催产素对子宫前列腺素合成的影响。 后续实验将 进行，以确定各种膜蛋白如何相互作用， 连接催产素受体和磷脂酶C。 这 信息将提供一个起点，从研究如何各种 激素和其他药剂改变催产素刺激 磷脂酶C活性。 第二个具体目标是确定如何 细胞内第二信使（即cAMP、甘油二酯、肌醇 三磷酸盐，Ca++）调节子宫PGF 2a的合成。 曾经重要的 确定第二信使后，将进行实验， 表征介导第二种免疫应答的特异性调节蛋白， 信使效应（即蛋白激酶，钙调蛋白）和靶点 其活性（即脂皮质素、磷脂酶A2、环氧合酶）。 我们对这些机制的理解的提高有可能 用于产科、避孕和治疗不孕症 以及食用家畜的生殖管理。

Uterine prostaglandins (PGs) play an essential role in implantation, parturition, luteolysis and maternal recognition of pregnancy. Yet, the factors regulating uterine synthesis of PGs are poorly understood. The objective of the experiments described in this proposal is to characterize the intracellular mechanisms which regulate this synthesis. Oxytocin stimulates synthesis of PGs from uterine tissue. The experiments described have two specific aims. The first is to examine the role of phospholipase C in mediating the stimulatory effect of oxytocin on uterine synthesis of PGs. Subsequent experiments will be conducted to determine how various integral membrane proteins interact to link the oxytocin receptor to the phospholipase C enzyme. This information will provide a starting point from which to study how various hormones and other agents modify the ability of oxytocin to stimulate activity of phospholipase C. The second specific aim is to determine how intracellular second messengers (i.e. cAMP, diacylglycerol, inositol triphosphate, Ca++) regulate uterine synthesis of PGF 2a. Once important second messengers are identified, experiments will be conducted to characterize the specific regulatory proteins which mediate the second messenger effects (i.e. protein kinases, calmodulin) and the targets of their activity (i.e. lipocortin, phospholipase A2, cyclooxygenase). Improvements in our understanding of these mechanisms has the potential for application in obstetrics, contraception and treatment of infertility and in the reproductive management of food producing livestock.