ONTOGENY OF AIRWAY SMOOTH MUSCLE FUNCTION

气道平滑肌功能的个体发育

基本信息

项目摘要

Neonatal respiratory distress syndrome (RDS) may be complicated by a forma of unresolved lung injury referred to as bronchopulmonary dysplasia (BPD). Treatment of RDS is believed to be partially responsible for the emergence of BPD by mechanisms such as oxygen toxicity. Many studies suggest that BPD is characterized in part by reactive airway disease in addition to lung parenchymal damage. On the other hand, while bronchodilators are frequently employed in the routine management of these infants, other studies suggest that large-airway collapse may complicate BPD and that bronchodilators may at times worsen airway obstruction by promoting large airway relaxation. Thus, abnormal airway smooth muscle tone probably plays an important role in the symptoms of BPD. Herein are described studies on isolated airway smooth muscle from 6 groups of guinea pigs: 1) term newborns between 1-3 days of age, randomized to breathe room air for 2-3 days; 2) or to breathe 95% oxygen for 2-3 days; 3) 6 week old adults (controls); 4) 6 week old adults that have been exposed to 95% oxygen in the newborn period for 2-3 days; 5) preterm newborns delivered 3-6 days prior to term and allowed to breathe room air; and 6)preterm newborns exposed to 95% oxygen as described for groups #2. These experiments will first define the normal ontogeny of airway smooth muscle responses to physiologic and pharmacologic stimuli in order to test the overall hypothesis that exposure to high oxygen concentrations during the newborn period alters the normal developmental pregression of airway smooth muscle function. The technique of isometric force measurements on segments of tracheal and bronchial rings will be employed to determine the following specific aims: 1) Determine if the normal ontogeny of airway smooth muscle response sis altered after exposure to high oxygen concentrations during the newborn period to physiologic, pharmacologic and environmental stimuli; 2) Determine if the effect of airway epithelial function during normal development changes after high oxygen exposure during the newborn period; 3) Determine if gestational age at birth influences airway smooth muscle reactivity following exposure to high oxygen concentrations; and 4) Determine if relaxation responses mediated by cAMP and cGMP correlate with measurements of intracellular cAMP and cGMP accumulation. The importance of these studies is best expressed by a recent NHLBI workshop summary on "Postnatal lung development in health and disease" that recommended that the "sequence of events leading to the early development of chronic lung diseases in neonates, . . . needs to be defined. The relative importance of oxygen toxicity, . . . and airway reactivity as etiologic factors in this disease process must be examined." (30). Thus, results of studies proposed herein hold the promise of achieving new knowledge that the NHLBI feel should be "given the highest priority" (30).
新生儿呼吸窘迫综合征 (RDS) 可能因以下原因而变得复杂 未解决的肺损伤称为支气管肺发育不良(BPD)。 RDS 的治疗被认为是导致 RDS 出现的部分原因 通过氧毒性等机制来治疗 BPD。 许多研究表明 除肺部疾病外,BPD 的部分特征还包括反应性气道疾病 实质损伤。 另一方面,虽然支气管扩张剂 经常用于这些婴儿的日常管理,其他 研究表明,大气道塌陷可能会使 BPD 复杂化,并且 支气管扩张剂有时可能会通过促进大的气道阻塞而加重气道阻塞。 气道松弛。 因此,异常的气道平滑肌张力可能会发挥作用 在 BPD 症状中起重要作用。 这里描述的研究 6组豚鼠离体气道平滑肌:1)术语 1-3 天大的新生儿,随机呼吸室内空气 2-3 次 天; 2)或呼吸95%氧气2-3天; 3) 6周大的成人 (控制); 4) 已暴露于 95% 氧气的 6 周龄成人 新生儿期2-3天; 5)早产新生儿出生3-6天 临产前并允许呼吸室内空气; 6) 早产儿 如第 2 组所述,暴露于 95% 氧气。 这些实验将 首先定义气道平滑肌反应的正常个体发育 生理和药理刺激,以测试整体 假设新生儿时期暴露于高浓度氧气 周期改变气道平滑肌的正常发育进程 功能。 等距力测量技术 气管和支气管环将用于确定以下内容 具体目的: 1)确定气道平滑肌个体发育是否正常 暴露于高浓度氧气后反应发生改变 新生儿期对生理、药理和环境刺激的影响; 2) 确定正常时气道上皮功能的影响 新生儿期高氧暴露后的发育变化; 3) 确定出生胎龄是否影响气道平滑肌 暴露于高浓度氧气后的反应性;和 4) 确定 cAMP 和 cGMP 介导的松弛反应是否与 细胞内 cAMP 和 cGMP 积累的测量。 重要性 最近的 NHLBI 研讨会总结很好地表达了这些研究的内容 “健康和疾病中的产后肺部发育”建议 “导致慢性肺病早期发展的一系列事件 新生儿疾病,. 。 。需要定义。 相对重要性 氧中毒,. 。 。和气道反应性作为病因 必须检查这种疾病过程。”(30)。因此,研究结果 本文提出的内容有望获得 NHLBI 所掌握的新知识 感觉应该“给予最高优先级”(30)。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KENNETH T NAKAMURA其他文献

KENNETH T NAKAMURA的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KENNETH T NAKAMURA', 18)}}的其他基金

ONTOGENY OF AIRWAY SMOOTH MUSCLE FUNCTION
气道平滑肌功能的个体发育
  • 批准号:
    3473252
  • 财政年份:
    1990
  • 资助金额:
    $ 8.06万
  • 项目类别:
ONTOGENY OF AIRWAY SMOOTH MUSCLE FUNCTION
气道平滑肌功能的个体发育
  • 批准号:
    2222009
  • 财政年份:
    1990
  • 资助金额:
    $ 8.06万
  • 项目类别:
ONTOGENY OF AIRWAY SMOOTH MUSCLE FUNCTION
气道平滑肌功能的个体发育
  • 批准号:
    3473255
  • 财政年份:
    1990
  • 资助金额:
    $ 8.06万
  • 项目类别:
ONTOGENY OF AIRWAY SMOOTH MUSCLE FUNCTION
气道平滑肌功能的个体发育
  • 批准号:
    3473253
  • 财政年份:
    1990
  • 资助金额:
    $ 8.06万
  • 项目类别:
SYMPATHETIC CONTROL OF RENAL FUNCTION DURING DEVELOPMENT
发育过程中肾功能的交感神经控制
  • 批准号:
    3081273
  • 财政年份:
    1985
  • 资助金额:
    $ 8.06万
  • 项目类别:
SYMPATHETIC CONTROL OF RENAL FUNCTION DURING DEVELOPMENT
发育过程中肾功能的交感神经控制
  • 批准号:
    3081272
  • 财政年份:
    1985
  • 资助金额:
    $ 8.06万
  • 项目类别:
SYMPATHETIC CONTROL OF RENAL FUNCTION DURING DEVELOPMENT
发育过程中肾功能的交感神经控制
  • 批准号:
    3081274
  • 财政年份:
    1985
  • 资助金额:
    $ 8.06万
  • 项目类别:
FEASIBILITY OF STUDYING THE PHYSIOLOGY AND PHARMACOLOGY OF HUMAN FETAL AIRWAY
研究人类胎儿气道生理学和药理学的可行性
  • 批准号:
    3745154
  • 财政年份:
  • 资助金额:
    $ 8.06万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了