GROWTH-ASSOCIATED PROTEIN-(GAP)-43 IN NEURONAL DEVELOPME

神经元发育中的生长相关蛋白-(GAP)-43

• 批准号: 3477603
• 负责人: Lawrence Baizer
• 金额: $ 10.45万
• 依托单位: GOOD SAMARITAN HOSP & MED CTR(PRTLND,OR)
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3477603
• 关键词: antiserum; axon; cell differentiation; chickens; gene expression; genetic promoter element; growth cones; immunocytochemistry; laboratory rabbit; membrane proteins; messenger RNA; molecular cloning; molecular weight; neoplastic cell culture for noncancer research; nervous system regeneration; neural growth associated protein; neurogenesis; neurotrophic factors; nucleic acid sequence

Growth Associated Protein-(GAP)-43 is an axonal protein expressed at elevated levels during periods of axonal growth and regeneration which appears to play a central role in these processes. Experiments outlined herein are directed towards analyzing the time-course and distribution of GAP-43, cloned into an expression vector, will be used to generate specific antisera. Immunocytochemical detention of GAP-43 in the developing nervous system will reveal when GAP-43 expression begins and hence whether this protein is involved in the early events of neurogenesis. Previous investigations have demonstrated that elevated expression of GAP-43 coincides with nerve growth factor (NGF)-induced neurite outgrowth from cultured PC12 pheochromocytoma cells. To assess the role of GAP-43 in NGF-induced neurite outgrowth, the cDNA for GAP- 43 under the control of an inducible promoter will be introduced into PC12 cells, thus permitting experimental modulation of the expression of this protein. These studies will reveal whether elevated expression of GAP-43 is sufficient or necessary for axonal outgrowth in this system. GAP-43 appears to be a member of a small group of proteins specifically involved in axonal growth and regeneration. Investigation of these proteins and the regulation of their expression may eventually permit us to promote axonal re-growth after injury to the nervous system.

与生长相关的蛋白质（GAP）-43是表达的轴突蛋白 在轴突生长和再生期间的水平升高处 这似乎在这些过程中起着核心作用。 本文概述的实验用于分析 GAP-43的时间课程和分布，克隆到表达式 向量将用于生成特定的抗血清。 GAP-43的免疫细胞化学拘留在发育中的神经 系统将揭示GAP-43表达式开始何时开始，因此是否是否 该蛋白参与神经发生的早期事件。 先前的研究表明表达升高 GAP-43与神经生长因子（NGF）诱导的神经突一致 培养的PC12嗜铬细胞瘤细胞的生长。 评估 GAP-43在NGF诱导的神经突生长中的作用，间隙的cDNA 43将在诱导型启动子的控制下被引入 进入PC12细胞，从而允许对 该蛋白的表达。 这些研究将揭示是否 GAP-43的表达升高是轴突足够或必需的 该系统中的生长。 GAP-43似乎是一小部分蛋白质的成员 特别参与轴突生长和再生。 研究这些蛋白质及其调节 表达最终可能使我们能够促进轴突重新生长 受伤后神经系统。