GUSTATORY SYSTEM--CELLULAR AND SYNAPTIC ORGANIZATION

关于孤束核细胞类型的知识，他们的 连接和突触关系，是 了解哺乳动物味觉的基本组织 系统。目前，关于细胞和突触的信息 孤立核的组织非常有限。 拟议的研究将提供有关 从最近的一次实验看孤立核的结构组织 在仓鼠体内对其神经元结构的重新评估。这个 从舌头接受初级传入输入的神经元类型 将研究与辣根过氧化物酶(HRP)标记相结合 用电子显微镜观察。内在与外在的联系 孤束核的细胞构筑的细分将 使用轴突运输/充盈方法识别。这个 方法包括点状注射放射性物质 氨基酸或辣根过氧化物酶(HRP) 孤束核的分支，或在脑干网状结构 味觉的形成、口面部运动核团或更高的中心 小路。辣根过氧化物酶标记投射神经元的形态观察 将与高尔基受孕的数据相关联，以确定 形成这些连接的细胞类型。这些研究还将 用电子显微镜分析其类型和分布 突触末梢汇聚在充满HRP的投射细胞上。通过 分析具有已知投影的细胞的结构细节， 这项研究将是定义突触的重要一步 脑干味觉系统的组织。来自这些网站的数据 研究应该允许提出假设是如何 孤核的电路处理和传播 味觉信息。颈椎的解剖学知识 味觉系统是评估味觉障碍所必需的 他们的预测。

Knowledge of the cell types of the solitary nucleus, their connections and synaptic relationships, is essential for understanding the basic organization of the mammalian gustatory system. At present, information about the cellular and synaptic organization of the solitary nucleus is very limited. The proposed research will provide detailed information about the structural organization of the solitary nucleus in light of a recent reevaluation of its neuronal architecture in the hamster. The neuron types that receive primary afferent inputs from the tongue will be studied horseradish peroxidase (HRP) labelling combined with electron microscopy. The intrinsic and extrinsic connections of the cyto-architectonic subdivisions of the solitary nucleus will be identified using axonal transport/filling methods. The approach involves making punctate injections of radioactive amino acids or horseradish peroxidase (HRP) in different subdivisions of the solitary nucleus, or in the brain stem reticular formation, orofacial motor nuclei, or higher centers of the taste pathways. The morphologies of HRP-labelled projection neurons will be correlated with data from Golgi impregnations to identify the cell types that form these connections. The studies will also employ electron microscopy to analyze the types and distributions of synaptic endings converging on HRP-filled projection cells. By analyzing the structural details of cells with known projections, this study will be an important step in defining the synaptic organization of the brain stem gustatory system. Data from these studies should allow the formulation of hypotheses about how the circuitry of the solitary nucleus processes and disseminates gustatory information. Knowledge of the anatomy of the gustatory system is required for evaluation of taste disorders and their prognoses.