GUSTATORY SYSTEM--CELLULAR AND SYNAPTIC ORGANIZATION

味觉系统——细胞和突触组织

• 批准号: 3477261
• 负责人: MARK Celestin WHITEHEAD
• 金额: $ 8.94万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3477261
• 关键词: afferent nerve; axon; brain stem; cell type; chorda tympani; cranial nerves; electron microscopy; hamsters; histology; nerve endings; neural information processing; neuroanatomy; neuronal transport; nontherapeutic iontophoresis; reticular formation; silver impregnation; solitary tract nucleus; synapses; taste; trigeminal nerve

Knowledge of the cell types of the solitary nucleus, their connections and synaptic relationships, is essential for understanding the basic organization of the mammalian gustatory system. At present, information about the cellular and synaptic organization of the solitary nucleus is very limited. The proposed research will provide detailed information about the structural organization of the solitary nucleus in light of a recent reevaluation of its neuronal architecture in the hamster. The neuron types that receive primary afferent inputs from the tongue will be studied horseradish peroxidase (HRP) labelling combined with electron microscopy. The intrinsic and extrinsic connections of the cyto-architectonic subdivisions of the solitary nucleus will be identified using axonal transport/filling methods. The approach involves making punctate injections of radioactive amino acids or horseradish peroxidase (HRP) in different subdivisions of the solitary nucleus, or in the brain stem reticular formation, orofacial motor nuclei, or higher centers of the taste pathways. The morphologies of HRP-labelled projection neurons will be correlated with data from Golgi impregnations to identify the cell types that form these connections. The studies will also employ electron microscopy to analyze the types and distributions of synaptic endings converging on HRP-filled projection cells. By analyzing the structural details of cells with known projections, this study will be an important step in defining the synaptic organization of the brain stem gustatory system. Data from these studies should allow the formulation of hypotheses about how the circuitry of the solitary nucleus processes and disseminates gustatory information. Knowledge of the anatomy of the gustatory system is required for evaluation of taste disorders and their prognoses.

了解孤核的细胞类型， 连接和突触关系，是必不可少的， 了解哺乳动物味觉的基本结构 系统 目前，关于细胞和突触的信息 孤核的组织非常有限。 拟议的研究将提供有关 孤核的结构组织，根据最近的 重新评估其在仓鼠的神经元结构。 的 接受来自舌头的初级传入输入的神经元类型 将辣根过氧化物酶（HRP）标记法结合 用电子显微镜。 内在和外在的联系 孤核的细胞结构细分将 使用轴突运输/填充方法进行鉴定。 的 这种方法包括将放射性药物 氨基酸或辣根过氧化物酶（HRP）在不同的 孤核的分支，或脑干网状 形成，口面运动核，或更高的味觉中心 途径。 HRP标记投射神经元的形态学 将与来自高尔基体的数据相关联， 形成这些连接的细胞类型。 这些研究还将 用电镜分析了其类型和分布 突触末端聚集在充满HRP的投射细胞上。 通过 分析具有已知投影的细胞的结构细节， 这项研究将是定义突触的重要一步， 脑干味觉系统的组织。 数据从这些 研究应允许制定关于如何 孤核的回路进行加工和散布 味觉信息 解剖学知识 味觉系统需要用于评价味觉障碍， 他们的屁股。