NORMAL AND ABNORMAL CELL SURFACE GENETICS

正常和异常细胞表面遗传学

• 批准号: 3479140
• 负责人: EDWARD A BOYSE
• 金额: $ 87.85万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-30 至 1992-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3479140
• 关键词: immunogenetics; neoplasm /cancer genetics; surface antigens

The title of this application comes from a review written in 1969 (84) and refers to the field of research which concerns itself with relating the genome as a whole to the composition of cell surfaces, principally on the initial basis of antigenic systems defined on normal or malignant cells by serological immunogenetics. The period covered in the narrative begins in 1957 when the only pertinent system known except for blood groups was H-2, itself first serologically defined as a blood group. During the next decade it became clear from the new systems described that the serological antigenic composition of tumor cell surfaces includes three categories, representing cellular genes expressed selectively by the cell lineage to which a given tumor belongs, cellular genes abnormally expressed by tumor cells, and partially or completely expressed retroviral genomes. In apparent contradiction of transplantation theory of the times, the new systems all displayed selective expression according to cell lineage. An account is given of how the systems that comprise T cell programs for surface phenotypes eventuated in applications to theoretical and practical immunology. Arguments stemming from the generality of lineage-specific cell surface phenotypes and inferred antiquity of the determining programs are presented in the contexts of development and phylogeny. Contrary to principles of functional differentiation, induction of expression of the multigene program for thymocyte surface phenotype is found to occur in vitro prior to cell division. Theoretical and practical implications of such induction assay methods with respect to hormonal and other inducers and to development in general are indicated, together with notes on the special place of the Tla system in this and other gene- regulatory situations that include irregular expression by leukemias. The systems used for illustration in the text are chosen for their particular value or promise and with a view to signifying applications of molecular biology that are closest to the central aims of this laboratory in understanding the physiology and pathology of cell surface phenotypes. In that connection a point is made that while biochemistry and cellular immunology, and latterly molecular biology, continue to be vital adjuncts, something more may be required before the potentially crucial aspect of supramolecular presentation can be practically assessed.

本申请的标题来自一篇写于1969年（84）的评论， 指的是研究领域，它关注的是将 基因组作为一个整体的细胞表面的组成，主要是在 在正常或恶性细胞上定义的抗原系统的初始基础， 血清免疫遗传学 叙述中所涵盖的时期始于1957年，当时唯一相关的 除了血型之外，已知的系统是H-2，它本身首先是血清学上的 定义为血型。 在接下来的十年里， 新的系统描述了肿瘤的血清学抗原组成， 细胞表面包括三类，代表细胞基因 由给定肿瘤所属的细胞谱系选择性表达， 肿瘤细胞异常表达的细胞基因，以及部分或 完全表达逆转录病毒基因组。 在移植理论明显矛盾的时代， 系统均显示根据细胞谱系的选择性表达。 一个 说明了包括T细胞程序的系统如何用于 在理论和实际应用中发生的表面表型 免疫学 起源于谱系特异性细胞的一般性的争论 表面表型和推断古代的决定程序是 在发展和生殖的背景下提出。 与功能分化的原则相反， 发现胸腺细胞表面表型的多基因程序的表达 在细胞分裂之前就发生了。 理论和实践 这种诱导测定方法对于激素和 其他诱导剂和一般的发展，以及 注意到Tla系统在这个和其他基因中的特殊位置- 包括白血病的不规则表达的调节情况。 文本中用于插图的系统是根据其 特定的价值或承诺，并旨在表明 分子生物学最接近这个实验室的中心目标， 了解细胞表面表型的生理和病理。 在 这种联系提出了一个观点，即生物化学和细胞 免疫学和后来分子生物学仍然是至关重要的学科， 在潜在的关键方面之前， 可以实际评估超分子呈现。