NORMAL AND ABNORMAL CELL SURFACE GENETICS

正常和异常细胞表面遗传学

• 批准号: 3479141
• 负责人: EDWARD A BOYSE
• 金额: $ 53.38万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-30 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3479141
• 关键词: immunogenetics; neoplasm /cancer genetics; surface antigens

The title of this application comes from a review written in 1969 (84) and refers to the field of research which concerns itself with relating the genome as a whole to the composition of cell surfaces, principally on the initial basis of antigenic systems defined on normal or malignant cells by serological immunogenetics. The period covered in the narrative begins in 1957 when the only pertinent system known except for blood groups was H-2, itself first serologically defined as a blood group. During the next decade it became clear from the new systems described that the serological antigenic composition of tumor cell surfaces includes three categories, representing cellular genes expressed selectively by the cell lineage to which a given tumor belongs, cellular genes abnormally expressed by tumor cells, and partially or completely expressed retroviral genomes. In apparent contradiction of transplantation theory of the times, the new systems all displayed selective expression according to cell lineage. An account is given of how the systems that comprise T cell programs for surface phenotypes eventuated in applications to theoretical and practical immunology. Arguments stemming from the generality of lineage-specific cell surface phenotypes and inferred antiquity of the determining programs are presented in the contexts of development and phylogeny. Contrary to principles of functional differentiation, induction of expression of the multigene program for thymocyte surface phenotype is found to occur in vitro prior to cell division. Theoretical and practical implications of such induction assay methods with respect to hormonal and other inducers and to development in general are indicated, together with notes on the special place of the Tla system in this and other gene- regulatory situations that include irregular expression by leukemias. The systems used for illustration in the text are chosen for their particular value or promise and with a view to signifying applications of molecular biology that are closest to the central aims of this laboratory in understanding the physiology and pathology of cell surface phenotypes. In that connection a point is made that while biochemistry and cellular immunology, and latterly molecular biology, continue to be vital adjuncts, something more may be required before the potentially crucial aspect of supramolecular presentation can be practically assessed.

本申请的标题来自于1969年(84年)写的一篇评论 指的是本身涉及到与 基因组作为一个整体到细胞表面的组成，主要是在 在正常或恶性细胞上定义的抗原系统的初步基础 血清免疫遗传学。 叙述中所涉及的时期始于1957年，当时唯一相关的 除了血型之外，已知的系统是H-2，它本身就是第一个血清学上的 定义为一个血型。在接下来的十年里，从 新的系统描述了肿瘤的血清抗原成分 细胞表面包括三类，代表细胞基因 通过特定肿瘤所属的细胞谱系选择性地表达， 肿瘤细胞异常表达的细胞基因，部分或 完全表达逆转录病毒基因组。 在明显矛盾的时代移植理论中，新的 所有系统均根据细胞谱系表现出选择性表达。一个 给出了组成T细胞程序的系统如何 表面表型在理论和实际应用中的最终结果 免疫学。起源于谱系特异性细胞的一般性的争论 测定程序的表面表型和推断的古代性是 在发展和系统发展的背景下提出。 与功能分化原则相反，诱导 胸腺细胞表面表型多基因程序的表达 在细胞分裂之前发生在体外。理论与实践 这种诱导试验方法对激素和 指出了其他诱因和一般发展因素，以及 关于Tla系统在该基因和其他基因中的特殊地位-- 调节情况，包括白血病的异常表达。 在正文中用于说明的系统被选择用于其 特定的价值或承诺，并以此为标志应用 分子生物学最接近这个实验室的中心目标 了解细胞表面表型的生理学和病理学。在……里面 这种联系的一个观点是，当生物化学和细胞 免疫学，以及最近的分子生物学，仍然是至关重要的辅助学科， 在潜在的关键方面之前，可能需要更多的东西 超分子呈现可以被实际评估。