DEVELOPMENT OF A RIA FOR HUMAN ERYTHROPOIETIN

人类促红细胞生成素 RIA 的开发

• 批准号: 3500911
• 负责人: ROBERT J WEBBER
• 金额: $ 5万
• 依托单位: RESEARCH AND DIAGNOSTIC ANTIBODIES
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-15 至 1987-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3500911
• 关键词: antibody; antibody specificity; antiserum; biotechnology; blood disorder diagnosis; diagnostic tests; erythropoietin; immunologic assay /test; peptide chemical synthesis; peptide hormone analog; radioimmunoassay; radiopharmacology; radiotracer; synthetic antigens; synthetic peptide

Erythropoietin (Epo) is a hormone produced by the kidneys and/or liver which determines the number of red blood cells produced. Recently, the nucleotide sequence of a cDNA which encodes this glycoprotein hormone has been determined. From this and from the amino acid sequence of tryptic peptides the complete amino acid sequence of the protein has been deduced. The goal of this project is to synthesize peptide analogs of various regions of the Epo amino acid sequence, and to use these peptide analogs as antigens for the production of antisera which can be used to develop a RIA for Epo. An immunoassay developed by this procedure would not require pure erythropoietin, but would only require the production of a pure peptide analog, which can by synthesized very economically on a large scale. The RIA which is to be developed during this project will be commercially available to physicians, clinical laboratories, and researchers for the diagnosis of patients with erythropoietic disorders and for monitoring the treatment of patients with various kidney disorders.

促红细胞生成素（Epo）是一种由肾脏和/或肝脏产生的激素