DEVELOPMENT OF METHODS FOR LARGE SCALE PEPTIDE SYNTHESIS

大规模肽合成方法的开发

• 批准号: 3638337
• 负责人: ROBERT J WEBBER
• 金额: $ 5万
• 依托单位: PEPTIDE LABORATORY, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3638337
• 关键词: analytical method; peptide chemical synthesis; peptide hormone

The objective of this project is to synthesize and purify 0.4 mole or more of the peptide hormone atrial natriuretic peptide (ANP). This large scale solid phase peptide synthesis would be the first attempt to mass synthesize a peptide greater than 10 amino acids. Specifically, this project is aimed at developing the large quantity of the solid support resin which is needed for teh Fmoc peptide synthesis; preparing and analyzing a large quantity of Fmoc- tyrosine (otBu; and derivatizing the resin with the carboyl terminal amino acid of ANP, tyrosine.

本课题的目标是合成并纯化0.4摩尔 或更多的肽激素心房利钠肽（ANP）。 这种大规模的固相肽合成将是第一个 尝试大量合成大于10个氨基酸的肽。 具体而言，该项目旨在开发大量 Fmoc肽所需的固体支持树脂 合成，制备并分析了大量的Fmoc- 酪氨酸（otBu）;和用羰基衍生化树脂 ANP末端氨基酸酪氨酸。