DECIDUAL PROLACTIN IN NORMAL AND PATHOLOGIC PREGNANCIES

这项建议的总体目标是扩大我们对 人催乳素合成和释放的调控 正常早产和足月妊娠的蜕膜组织以及 妊娠合并糖尿病、子痫前期、羊水过多 以及其他病理情况。在过去的几年里，我们已经 证明了蜕膜合成和释放的调节 催乳素不同于垂体催乳素。无论是合成还是 蜕膜催乳素的释放似乎主要在“局部控制”之下， 受胎盘释放的一种肽的刺激和抑制 花生四烯酸和蜕膜释放的多肽。具体的 该提案的目的是(1)分离和鉴定胎盘和 影响蜕膜催乳素和催乳素释放的蜕膜肽 发展放射免疫分析方法来检测它们；(2)研究 多肽对蜕膜外植体合成和释放催乳素的影响 从正常和病理妊娠的不同阶段，(3) 研究孕酮、雌激素、前列腺素等的作用 影响两种多肽合成和释放的不同阶段因素 (4)确定蜕膜结合的数量和亲和力 在怀孕期间多肽的位置，以及(5)检查 多肽对环磷酸腺苷水平、磷脂酶A2活性的影响 富集组分中磷脂酰肌醇循环和钙通量 蜕膜细胞，合成和释放催乳素。自研究以来 说明蜕膜组织是大量 羊水中的催乳素，这些研究应该提供洞察力 正常与病理羊水催乳素调节的探讨 怀孕。这种检查具有临床意义，因为羊水 体液催乳素似乎有许多重要的生理作用， 包括调节羊水中的离子和水分含量。

The overall objective of this proposal is to extend our investigations of the regulation of the synthesis and release of prolactin from human decidual tissue from normal pre-term and term pregnancies and from pregnancies complicated by diabetes mellitus, pre-eclampsia, polyhydramnios and other pathologic conditions. During the past few years, we have demonstrated that the regulation of the synthesis and release of decidual prolactin differs from that of pituitary prolactin. Both the synthesis and release of decidual prolactin appear to be primarily under "local control, stimulated by a peptide released by the placenta and inhibited by arachidonic acid and by the peptide released by the decidua. The specific aims of the proposal are to (1) isolate and characterize the placental and decidual peptides which affect the release of decidual prolactin and develop radioimmunoassays for their detection, (2) study the effects of the peptides on the synthesis and release of prolactin from decidual explants from normal and pathologic pregnancies at various stages of gestation, (3) investigate the effects of progesterone, estrogen, prostaglandin and other factors on the synthesis and release of the two peptides at various stages of gestation, (4) determine the number and affinity of decidual binding sites for the peptides during pregnancy, and (5) examine the effects of the peptides on cyclic AMP levels, phospholipase A2 activity, the phosphatidylinositol cycle and calcium flux in an enriched fraction of decidual cells which synthesize and release prolactin. Since studies indicate that decidual tissue is the source of the large quantities of prolactin in amniotic fluid, these investigations should provide insight into the regulation of amniotic fluid prolactin in normal and pathologic pregnancies. Such investigations are of clinical importance since amniotic fluid prolactin appears to have many important physiologic actions, including regulation of the ion and water content of amniotic fluid.