DEVELOPMENT OF A CONTROLLED ENVIRONMENT CHAMBER

长期目标是开发一种机械装置， 将提供完全的环境控制（温度， 压力、湿度、无菌性、pH值、气体气氛和光照 射线）在显微镜下对生物或工业样品 考试 即使在下列期间，也将保持控制 对标本的物理操作和显微镜检查期间 通过设计中固有的光学透明透镜观察 的装置。 具体的目标是从一个 样机 方法将包括基于机械的设计 工程和物理原理，考虑到现有的 技术和对生物医学领域投入的反应 部门 将测试该器械是否能够保持 环境与当前的实验室技术， 活体组织培养物显微注射实验 细胞 没有一种技术可以提供完全的环境保护。 控制在一个经济的格式，可以很容易地管理， 显微镜的载物台。 商业应用，将 成功的研究结果包括增加 任何实验的准确性和效率， 环境控制，保护研究人员免受 有害物质的敌对生物， 人类体外受精成功率与能量消耗 石油蒸馏过程中的还原。

The long term objective is to develop a mechanical device that will provide complete environmental control (temperature, pressure, humidity, sterility, pH, gaseous atmosphere and light rays) to biological or industrial specimens under microscopic examination. Controls will be maintained even during periods of physical manipulation of the specimen and during microscopic observation through optically clear lenses inherent in the design of the device. The specific aim is to develop a functional device from a prototype. Methodology will include design based on mechanical engineering and physical principles, consideration of existent technology and responsiveness to input from the biomedical sector. The device will be tested as to its ability to maintain environment against current laboratory techniques with experiments involving the microinjection of living tissue culture cells. There is no technology that provides for complete environmental control in an economical format that can be easily managed on the stage of a microscope. Commercial applications that will result from successful research include an increase in the accuracy and efficiency of any experiment requiring environmental control, a means of protecting researchers from hostile organisms of noxious materials, an improvement in the success rate of human in vitro fertilization and energy cost reduction during petroleum distillation.