DEVELOPMENT OF A CONTROLLED ENVIRONMENT CHAMBER

长期目标是开发一种机械设备， 将提供完全的环境控制(温度、 压力、湿度、无菌、pH、气态大气和光照 射线)到显微镜下的生物或工业标本 考试。即使在以下期间也将保持控制 标本的物理操作和在显微镜下 通过设计中固有的光学透明透镜进行观察 该设备的。 其具体目标是开发一种功能设备，从一个 原型。方法论将包括基于机械的设计 工程和物理原理，对存在的考虑 技术和对来自生物医学的投入的响应 扇区。将对该设备进行测试，以确定其维护能力 环境与当前的实验室技术 活体组织培养的显微注射实验 细胞。 没有一种技术可以提供完全的环境保护 控件以经济实惠的格式，可在 显微镜的工作台。商业应用程序将 成功研究的结果包括增加了 任何需要的实验的准确性和效率 环境控制，一种保护研究人员免受 有害物质的敌意生物，改进了 人体体外受精成功率与能源成本 石油蒸馏过程中的还原。

The long term objective is to develop a mechanical device that will provide complete environmental control (temperature, pressure, humidity, sterility, pH, gaseous atmosphere and light rays) to biological or industrial specimens under microscopic examination. Controls will be maintained even during periods of physical manipulation of the specimen and during microscopic observation through optically clear lenses inherent in the design of the device. The specific aim is to develop a functional device from a prototype. Methodology will include design based on mechanical engineering and physical principles, consideration of existent technology and responsiveness to input from the biomedical sector. The device will be tested as to its ability to maintain environment against current laboratory techniques with experiments involving the microinjection of living tissue culture cells. There is no technology that provides for complete environmental control in an economical format that can be easily managed on the stage of a microscope. Commercial applications that will result from successful research include an increase in the accuracy and efficiency of any experiment requiring environmental control, a means of protecting researchers from hostile organisms of noxious materials, an improvement in the success rate of human in vitro fertilization and energy cost reduction during petroleum distillation.