HERPESVIRIDAE INFECTIONS & THE PROGRESSION OF AIDS

艾滋病是一种临床综合征，由 免疫系统中的病原体种类繁多。经典 进展是从接触艾滋病毒到血清转换，再到ARC，再到艾滋病。 对艾滋病毒以外的病原体进行准确诊断对于 确定疾病的进展和适当的治疗。 此外，有证据表明，病毒的相互作用会加速 从ARC发展到艾滋病和死亡。如果是这样的话，快速诊断 这些病毒是极其重要的。 直接与酶相连的DNA探针可以快速、容易和 有效地用于诊断和量化这些 病毒病原体，无论免疫反应的状态如何， 抗原掩蔽，或治疗干预。 在第一阶段，Digene的直接标记DNA探针将用于 用快速原位杂交技术检测病毒在人体内的存在 与艾滋病相关的疾病，如毛状白斑、卡波西病 肉瘤、其他皮肤病和肺炎。数量和数量 这些病毒的组织学位置将被调查。 第二阶段将通过研究发病机制来应用这些发现。 各种与艾滋病相关的疾病及其意义 疱疹病毒对HIV复制的影响确定各种抗病毒药物 治疗方法可以减少病毒复制。

AIDS is a clinical syndrome resulting from the interaction of a wide variety of pathogens with the immune system. The classic progression is from HIV exposure to seroconversion to ARC to AIDS. Accurate diagnosis of pathogens other than HIV is necessary to determine the progression of disease and appropriate therapy. Also, evidence suggests that viral interaction accelerates the progression from ARC to AIDS and death. If so, rapid diagnosis of these viruses os of extreme importance. DNA probes directly linked to enzymes can be quickly, easily, and effectively used to diagnose and quantify the presence of these viral pathogens, regardless of the status of the immune responses, antigen masking, or therapeutic intervention. In Phase I, DIGENE's directly labeled DNA probes will be used in rapid in situ hybridizations to detect the presence of viruses in AIDS-associated disorders, such as hairy leukoplakia, Kaposi's sarcoma, other skin disease, and pneumonia. The quantity and histologic location of these viruses will be investigated. Phase II would apply these findings by studying the pathogenesis of various AIDS-associated disorders and the significance of herpesviruses on HIV replication to determine if various antiviral therapies decrease viral replication.