IMPROVED CHROMOSOME ANALYSIS VIA NEW SYNCHRONIZING AGENT

通过新的同步剂改进染色体分析

• 批准号: 3493648
• 负责人: FLOYD E TAUB
• 金额: $ 5万
• 依托单位: SYNCHROCELL, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-13 至 1994-08-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3493648
• 关键词: cell cycle proteins; karyotype; method development; synchronous cell division

DESCRIPTION (Adapted from applicant's abstract): Traditional karyotypic analysis of cells requires metaphase spreads. Obtaining metaphase spreads is dependent upon obtaining cells in metaphase. Generally a relatively small number, frequently only three-five percent, of the cells are in metaphase. A cell regulatory sialoprotein (CeReS) has been demonstrated biochemically to synchronize better than 90 percent of the cells in culture and to lead to mitosis of nearly all the cells in the culture within a short time. Data suggesting the applicability of this material to a wide range of species and tissue types including human products of conception are presented. The goal of the study is to determine if use of CeReS to increase the number and quality of metaphase spreads is feasible. Synchro Cell, the exclusive licensee of CeReS, believes that the use of this molecule may increase speed and reduce cost of routine karyotypic analysis. In addition, prometaphase spreads providing detailed banding may be more readily obtained.Further sample types that previously exhibited too low a mitotic index may become amenable to routine analysis. This will further scientific investigation and may allow commercial clinical applications not before possible.

描述（改编自申请人摘要）：传统核型 细胞的分析需要中期扩散。 获得中期 扩散依赖于获得中期细胞。 通常 相对较少的数字，往往只有百分之三五， 细胞处于中期。 细胞调节性唾液酸蛋白（CeReS）已被证明 在生物化学上， 并导致培养物中几乎所有细胞的有丝分裂 在短时间内。 表明该材料适用性的数据 广泛的物种和组织类型，包括人类产品， 提出了概念。 该研究的目的是确定使用CeReS是否能增加 中期扩散的数量和质量是可行的。 同步细胞 CeReS的独家许可人认为，使用这种分子可能 提高了常规核型分析速度并降低了成本。在 此外，提供详细带型的前中期扩散可能更多， 容易获得。以前表现出太低的其他样品类型 有丝分裂指数可能变得适合于常规分析。 这将 进一步的科学研究，并可能允许商业临床 应用程序不可能在此之前。