ERYTHROPOIETIN--PURIFICATION, PROPERTIES, BIOGENESIS

促红细胞生成素——纯化、特性、生物发生

• 批准号: 3485742
• 负责人: EUGENE GOLDWASSER
• 金额: $ 41.95万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3485742
• 关键词: affinity chromatography; cell growth regulation; chemical structure; chemical structure function; erythropoiesis; erythropoietin; estrogen receptors; genetic manipulation; genetic translation; high performance liquid chromatography; hormone regulation /control mechanism; human tissue; iodine; laboratory mouse; laboratory rat; molecular cloning; molecular site; monoclonal antibody; nucleic acid probes; peptide hormone; peptide hormone biosynthesis; protein sequence; radioimmunoassay; radionuclides; radiotracer; receptor mediated endocytosis; secretion; tissue /cell culture; urinalysis

The salient features of this proposal are: The study of those structural features of human erythropoietin that may be directly involved with its biological activity on hemopoietic precursor cells; the study of the primary structure of the active site; the determination of oligosaccharide side chain structure; the isolation and characterization of specific cellular receptors for erythropoietin from cells infected with the anemia strain of the Friend virus; the extension of receptor studies to normal mouse cells and to apply the methods developed for that purpose to the study of human cellular receptors. Once isolated the erythropoietin receptor will be cloned and the gene for the receptor used to test a hypothesis regarding erythroid cell differentiation. Mouse erythropoietin will also be cloned and the DNA used for the study of the regulation of expression of the erythropoietin gene as well as for the study of erythropoietin biogenesis and secretion. Another aim is to develop a solid-phase radioimmunoassay using monoclonal anti-epo with the intention of improving the sensitivity of analyis by an order of magnitude. The same monoclonal anti-epo will be used as the basis for an improved immunoaffinity purification method.

本提案的显著特点是：对那些结构性的研究