CALCIUM TRANSIENTS IN STRIATED MUSCLE AND OTHER CELLS

横纹肌和其他细胞中的钙瞬变

• 批准号: 3485307
• 负责人: JOHN R BLINKS
• 金额: $ 26.03万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-04-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3485307
• 关键词: Anura; Mammalia; alpha adrenergic agent; bioluminescence; calcium binding protein; calcium channel; calcium metabolism; cats; dogs; gel electrophoresis; guinea pigs; ion exchange chromatography; laboratory rabbit; laboratory rat; muscle pharmacology; myocardium; saltwater environment; sarcoplasmic reticulum; striated muscles

The calcium-sensitive bioluminescent protein aequorin and related photoproteins will be used to study the regulation of intracellular Ca++ concentration in frog skeletal muscle, and in frog and mammalian cardiac muscle. Results will be compared in some circumstances with those obtained with calcium-selective micro- electrodes. High-gain microscopic image intensification with computer image processing will be used to localize Ca++ in aequorin-injected cells. A major effort will be mounted to resolve the isospecies of aequorin and other photoproteins, and to study their kinetics and Ca++-sensitivity. Particular emphasis in the studies on cardiac muscle will be given to the analysis of the role played by changes in myofibrillar responsiveness to Ca++ in responses of the heart to inotropic interventions, particularly the response to alpha-adrenoceptor stimulation. Systematic analysis will also be carried out of the factors responsible for alterations in the Ca++ transient after changes in muscle length and contraction frequency. In skeletal muscle a variety of experiments are planned to critically test the hypothesis that inositol triphosphate release is centrally involved in excitation:contraction coupling.

钙敏感生物发光蛋白Aequorin及其相关 光蛋白将被用于研究细胞内的调节 青蛙骨骼肌中的钙离子浓度，以及青蛙和 哺乳动物的心肌。结果将在一些情况下进行比较 与用钙选择微量获得的情况一致 电极。高增益显微图像增强技术 将使用计算机图像处理来定位Ca++在 注射Aequorin的细胞。将作出重大努力，以解决 Aequorin和其他光蛋白的同种，并对其进行研究 它们的动力学和对钙的敏感性。特别强调的是 对心肌的研究将给予作用的分析 肌原纤维对钙离子反应性的变化 心脏对正性肌力干预的反应，特别是 对α肾上腺素能受体刺激的反应。系统分析 也将对造成变更的因素进行分析 在钙离子瞬变后肌肉的长度和长度发生变化 收缩频率。在骨骼肌中进行了各种实验 计划对肌醇的假设进行批判性测试 三磷酸盐的释放主要参与了 激励：收缩联轴器。