ABUSED DRUG QUANTIFICATION BY EIA IN ALL BODY FLUIDS

通过 EIA 对所有体液中的滥用药物进行定量

• 批准号: 3494385
• 负责人: STANLEY J GROSS
• 金额: $ 5万
• 依托单位: IMMUNALYSIS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-30 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3494385
• 关键词: blood chemistry; body fluids; breath tests; cannabinoids; diagnosis design /evaluation; drug abuse; drug metabolism; enzyme linked immunosorbent assay; gas chromatography mass spectrometry; human subject; immunoconjugates; urinalysis

The objective is to quantify by spectral absorbance, the significant metabolites of behavior-modifying drugs in urine, blood (hemolyzed) and breath compartments using our relevant antibodies. Guidelines are to state that positives should be confirmed by GC-MS. In instances where GC-MS is not readily available, e.g. blood 9-THC, or at sites not always accessible to the main laboratory as in the late hour hospital emergency work, positives are called "suggestive" but not "definitive". Reliability sensitivity and accuracy have been achieved in prototype tests requiring minimal manipulation. Effective procedures are suited to instruments ranging from a battery-operated hand-held device (4" x 5 1/4 x 1/4") to advanced bench top components appropriate for automation without compromise in accuracy over a broad range from 2-1000 ng/ml. Detection of multiple discrete drug metabolites using single or multiple plastic strips permit processing of one to several hundred samples (urine, blood, breath) simultaneously in a single run. This is useful for random screening, rehabilitation study and for-cause drug detection. Purified IgGs are immobilized in microcontainers. Incubation therein of standard (calibrator) and enzyme drug conjugate is followed by wash and substrate addition. Color develops, absorbance determined and translated by microprocessor into nanograms/milli-liter. Portable and bench formats have been thoroughly tested. The latter can be coupled to automatic pipettors, printers, etc., designed to process samples readily in parallel not requiring completion of sample analysis in series with minimum hands-on time.

目的是通过光谱吸光度进行量化，显着 尿液、血液（溶血）和行为改变药物的代谢物 使用我们相关抗体的呼吸室。 指南应说明 阳性结果应通过 GC-MS 确认。 在 GC-MS 的情况下 不容易获得，例如血液 9-THC，或部位并不总是 在深夜医院紧急情况下可进入主实验室 在工作中，积极因素被称为“暗示性”，而不是“决定性”。 可靠性 在原型测试中已经达到了灵敏度和准确性，需要 最少的操纵。 有效的程序适合仪器 范围从电池供电的手持设备 (4" x 5 1/4 x 1/4") 到 适合自动化的先进台式组件，毫不妥协 准确度范围为 2-1000 ng/ml。 检测多个 使用单个或多个塑料条允许离散的药物代谢物 处理一到数百个样本（尿液、血液、呼吸） 在一次运行中同时进行。 这对于随机筛选很有用， 康复研究和原因药物检测。 纯化的 IgG 是 固定在微容器中。 标准品在其中孵化 （校准品）和酶药物缀合物，然后进行洗涤和底物 添加。 显色、吸光度确定并转化为 微处理器转换为纳克/毫升。 便携式和台式格式有 经过彻底测试。 后者可以连接到自动移液器， 打印机等，旨在轻松并行处理样品，而不是 需要以最少的动手能力完成系列样品分析 时间。