DNA PROBES FOR THE DETECTION OF LYME DISEASE

莱姆病的治疗可以大大提高，如果它是 可以直接在血液中检测莱姆病螺旋体， 高灵敏度测试 我们建议开发一个测试，其中一个区域的 B。使用PCR扩增伯氏菌ospA基因，以获得 必要的敏感性。 扩增产物将被初步检测 通过Southern印迹，并最终通过夹心杂交， 使用非放射性DNA探针微量滴定威尔斯孔。 在第一阶段，我们建议 开发B的PCR扩增所需的试剂。 如果可能的话，测试检测burgdorferi DNA的可行性， PCR和Southern杂交检测螺旋体。 一个直接而敏感的 这种类型的莱姆病测试应该允许早期发现，更好地 诊断确认和抗生素治疗的有效监测。

The treatment of Lyme disease could be greatly enhanced if it were possible to detect the lyme disease spirochete directly in blood, with a highly sensitive test. We propose to develop a test where a region of the B. burgdorderi ospA gene is amplified using the PCR to obtain the necessary sensitivity. The amplified product will be detected initially by Southern blotting and eventually by sandwich hybridization in microtiter wells using a nonradioactive DNA probe. In Phase I, we propose to develop the reagents necessary for the PCR amplification of B. burgdorferi DNA and if possible, to test the feasibility of detecting the spirochete using the PCR and Southern blotting. A direct and sensitive test of this type for Lyme disease should permit earlier detection, better diagnostic confirmation and effective monitoring of antibiotic therapy.