USE OF PCR FOR DIAGNOSING HIV-I INFECTION

使用 PCR 诊断 HIV-I 感染

• 批准号: 3489152
• 负责人: MARK M MANAK
• 金额: $ 5万
• 依托单位: BIOTECH RESEARCH LABORATORIES
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1991-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3489152
• 关键词: AIDS /HIV test; HIV infections; diagnosis design /evaluation; human immunodeficiency virus 1; human tissue; longitudinal human study; newborn human (0-6 weeks); nucleic acid hybridization; polymerase chain reaction; western blottings

Prevalence studies for HIV-1 infection in newborns are difficult to obtain since the commonly used antibody assays are not appropriate for testing babies. Babies carry the maternal antibody for up to 12-18 months after birth, so that all babies born to seropositive mothers will also test positive. Yet 40-50% of these babies are not actually infected. In this grant, we propose to use Polymerase Chain Reaction (PCR) assays to examine the lymphocytes of babies born to HIV-1 seropositive mothers for the presence of the HIV-1 genome. This is an extremely sensitive effect test for the virus, and is capable of detecting even 10 viral genomes in less than 1 ml of blood. Peripheral blood lymphocytes will be collected from about 80 babies born to HIV-1 seropositive mothers. Samples collected at birth and at 3-month follow-up intervals will be tested by PCR and Western Blot assays. The results of the PCR will be correlated with the subsequent loss of maternal antibody and seroconversion to baby antibody. These results will determine whether PCR data can be used as a reliable indicator of actual infection and a predictor of subsequent seroconversion. These studies will provide epidemiological data for the incidence of HIV-1 infection in babies born to seropositive mothers, and provide a basis for predictive tests for AIDS in these babies.

新生儿 HIV-1 感染率研究很难获得 因为常用的抗体测定法不适合测试 宝贝们。 婴儿在出生后长达 12-18 个月内携带母源抗体 出生，以便所有血清呈阳性的母亲所生的婴儿也将接受检测 积极的。 然而，其中 40-50% 的婴儿实际上并未受到感染。 在这个 授予，我们建议使用聚合酶链反应（PCR）检测来检查 HIV-1 血清阳性母亲所生婴儿的淋巴细胞 HIV-1 基因组的存在。 这是一个极其敏感的效果测试 对于病毒，甚至能够在更少的时间内检测 10 个病毒基因组 超过1毫升的血液。 外周血淋巴细胞将从 HIV-1 血清阳性母亲所生的大约 80 名婴儿。 样本采集于 出生后和每 3 个月的随访间隔将通过 PCR 和 Western 进行检测 印迹分析。 PCR的结果将与后续的相关 母体抗体丧失和血清转化为婴儿抗体。 这些 结果将决定PCR数据是否可以作为可靠的指标 实际感染和随后血清转化的预测因子。 这些 研究将提供 HIV-1 发病率的流行病学数据 血清阳性母亲所生婴儿的感染，并为 这些婴儿的艾滋病预测测试。