TOXICITY AND GROWTH INHIBITION ASSAYS BY IMAGE ANALYSIS

通过图像分析进行毒性和生长抑制测定

• 批准号: 3492919
• 负责人: MORRIS E. KAIGHN
• 金额: $ 5万
• 依托单位: BIOLOGICAL RESEARCH FACULTY & FACILITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3492919
• 关键词: computer data analysis; cytotoxicity; drug adverse effect; evaluation /testing; growth inhibitors; image processing

DESCRIPTION: (Adapted from the applicant's abstract) The ultimate objective of this project is to develop new methodologies to measure and distinguish between cytotoxicity and growth inhibition by toxic chemicals, carcinogenic agents and chemotherapeutic drugs. The specific aims include: (a) To establish the feasibility of measuring cell numbers in multiwell microculture plates by image analysis. (b) To validate the image analysis approach for (i) cytotoxicity testing using short term exposure to selected toxic substances, and (ii) growth inhibition at low cell density and continuous long-term exposure. (c) To apply computer-assisted acquisition and analysis of the image analysis data. The technical innovation in this project lies in the application of existing image analysis technology to the problem of direct measurement of cell numbers in situ in microwell culture dishes, thereby avoiding time-consuming, labor-intensive procedures designed to measure cell number by counting dispersed cell suspensions or by biochemical methods.

描述：（改编自申请人的摘要）最终 该项目的目标是开发新的方法来衡量和 区分毒性化学品的细胞毒性和生长抑制， 致癌剂和化疗药物。 具体目标包括： (a)建立多微孔中细胞数测量的可行性 通过图像分析的显微培养板。 (b)为了验证图像分析 （i）使用短期暴露于选定的 有毒物质，和（ii）在低细胞密度下的生长抑制， 持续长期暴露。 (c)应用计算机辅助采集 和图像分析数据的分析。 这方面的技术创新 项目在于应用现有的图像分析技术， 微孔中细胞数原位直接测量问题 培养皿，从而避免耗时、劳动密集型的程序 设计用于通过计数分散的细胞悬液来测量细胞数量，或 通过生物化学方法。