RAPID CHEMILUMINESCENT SCREEN TEST FOR MICROORGANISMS

微生物快速化学发光筛选测试

• 批准号: 3489402
• 负责人: ROBERT J CARRICO
• 金额: $ 3.74万
• 依托单位: SERIM RESEARCH CORPORATION
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3489402
• 关键词: aerobic bacteria; bacteriuria; bioluminescence; diagnosis design /evaluation; electron transport; free radical oxygen; mass screening; paraquat; rapid diagnosis; urinalysis

DESCRIPTION: (Adapted from the applicant's abstract) Culture of urine is the usual method for diagnosis of bacteriuria in clinical microbiology laboratories. About 65 million urine specimens are tested by this method per year in the U.S. Each specimen is plated on two media in order to detect important gram positive and gram negative results. The goal of the proposed project is to develop a rapid screen test that will identify infected and uninfected urines. Then the cost for diagnosis of bacteriuria can be reduced because only infected urines will be cultured for isolation and characterization of the bacteria. Preliminary experiments have shown that energy from normal cell metabolism can be diverted into chemiluminescent emitted light which can be related to the presence of microorganisms. The research plan proposes to investigate the mechanism of the chemiluminescent reaction and optimize assay performance. The work will use lucigenin and luminol as chemiluminescent probes and will use redox active agents, such as paraquat, to transfer reducing equivalents from enzymes and cofactors to oxygen and the chemiluminescent probes. An important goal will be to achieve assay performance that will allow the method to be applied to the diagnosis of bacteriuria.

描述：（改编自申请人的摘要）尿液培养是 临床微生物学诊断菌尿症常用方法 laboratories. 大约有6500万份尿液样本通过这种方法进行检测 每个标本都涂在两种培养基上， 检测重要的革兰阳性和革兰阴性结果。 的目标 拟议的项目是开发一个快速筛选测试，将确定 感染和未感染的尿液。 那么诊断的费用 菌尿可以减少，因为只有受感染的尿液将被培养 用于细菌的分离和表征。 初步 实验表明，来自正常细胞代谢的能量可以被 被转移到发光的发射光中，其可以与 微生物的存在。 研究计划建议调查 荧光反应的机理和优化分析性能。 这项工作将使用光泽精和鲁米诺作为荧光探针， 将使用氧化还原活性剂，如百草枯， 从酶和辅因子到氧的当量， probes. 一个重要的目标是实现检测性能， 使该方法能够应用于菌尿症的诊断。