IMMUNOCHROMATOGRAPHY ASSAY FOR MICROALBUMINURIA

微量白蛋白尿是早期肾病发作的标志， 影响35%的I型糖尿病患者。 早期肾病 发展到终末期肾病。 的成本 末期肾病的糖尿病患者的护理费用约为750美元 每年百万。 早期血糖调节和治疗干预 可以减缓或阻止早期肾病向终末期的进展 肾脏疾病 目的是建立一种半定量免疫分析方法 因为尿中白蛋白含量低 该分析可以在医生的 办公室和病人在家里。 该测定基于 以尿液为展开液的免疫层析法。 竞争 将评价结合免疫测定形式。 抗人白蛋白抗体 将被固定在微孔膜如硝化纤维素上， 这将是色谱支持介质。 彩色乳胶标签将 用人血白蛋白包被，并将在 层析条 尿样会接触到有色乳胶 它将随着尿液迁移到试纸条上。 免疫结合 将捕获条上的有色乳胶并创建有色带。 的 将目视读取色带的位置，并与 白蛋白浓度

Microalbuminuria is a marker for the onset of incipient nephropathy, a condition that affects 35% of Type I diabetics. Incipient nephropathy develops over a period of years into end stage renal disease. The cost of care for diabetic patients with end stage renal disease is about $750 million per year. Early glycemic regulation and therapeutic intervention can slow or halt the progress of incipient nephropathy toward end stage renal disease. The objective is to develop a semiquantitative immunoassay for low levels of albumin in urine. The assay can be performed in doctors' offices and by patients at home. The assay is based on immunochromatography with urine as the developing liquid. A competitive binding immunoassay format will be evaluated. Antibody to human albumin will be immobilized on a microporous membrane such as nitrocellulose and this will be the chromatography support medium. A colored latex label will be coated with human albumin and will be dried near the bottom of the chromatography strip. The urine sample will contact the colored latex first and it will migrate with the urine onto the strip. Immunobinding will capture the colored latex on the strip and create a colored band. The position of the colored band will be read visually and related to the albumin concentration.