METHOD AND APPARATUS FOR LARGE SCALE DNA SYNTHESIS

描述（改编自申请人的摘要）：目的是开发一种 化学合成50-500克的简单、低成本的手段， 可用于反义DNA的寡核苷酸的量 治疗和诊断。 研究目的是调查 改进的化学物质，组装一个手动装置， 固相氰乙基亚磷酰胺或膦酸酯化学， 在合成OS 15-25残基序列中测试该系统。 的 研究将涉及开发（a）一种新的固体载体或一种新的 处理大量现有固体载体的方法，和（B） 一种回收昂贵的DNA双链体的方法， 大量过量以使逐步产量最大化。 高性能 逐步产率在小规模商业仪器中是常规的， 一个重要的特点，为更大规模的合成，以简化 后续纯化步骤。 的潜在应用 研究是为了快速和经济地制备大量的 这些寡核苷酸对于临床测试或最终的 治疗药物或诊断试剂的制造。这些 发展将是经济方面的重要成就， 竞争力 改进的方法，克量的化学 人工合成DNA将加速药物开发，降低成本， 生物技术产品的医疗应用。

DESCRIPTION (Adapted from applicant's abstract):The aim is to develop a simple, low cost means for the chemical synthesis of 50-500 gram quantities of oligonucleotides which can be utilized for antisense-DNA therapeutics and diagnostics. The research objectives are to investigate improved chemistries, to assemble a manual device which will utilize solid-phase cyanoethyl phosphoramidite or phosphonate chemistries and to test the system in the synthesis os 15-25 residue sequences. The research will involve the development of (a) a new solid support or a new means of handling large amounts of the existing solid supports and (b) a means to recycle the costly DNA synthons so that they can be used in large excess to maximize stepwise yields. The capability of high stepwise yields is routine in small scale, commercial instruments and is an important feature for larger scale synthesis in order to simplify the subsequent purification steps. The potential applications of the research are for the rapid and economical preparation of quantities of oligonucleotide that are necessary for clinical testing or for eventual manufacturing of therapeutic drugs or diagnostic reagents. These developments will be important achievements in terms of economic competitiveness. Improved methods for gram amounts of chemically synthesized DNA will accelerate drug development, reduce cost and enhance medical applications of biotechnology products.