Novel Chromatographic Sorbents for Large Biomolecules

描述(申请人提供)：该项目致力于开发一种新型的色谱介质，通过在非织造布上涂覆非常薄的聚合物涂层并具有适合吸附和纯化大生物分子的离子交换基团来制备。由此产生的色谱介质和设备将具有高效率和坚固性的组合，使它们的使用比用于类似应用的现有介质更有效、更高效、成本更低。其具体目标是(1)优化和开发具有离子交换功能的涂层非织造布系列的化学成分，(2)使用用于连续涂覆非织造布片材的设备开发和演示涂层工艺的性能，(3)表征应用于重组蛋白质的底物，(4)基于涂层非织造布的特性建立原型设备并表征性能。生物大分子的提纯是生物学研究的重要组成部分，也是生产用于多种医学用途的重组蛋白的重要组成部分。用于这一目的的传统色谱介质具有大的孔来容纳这些大分子。因此，它们是易碎和压缩的，这使得它们在柱层析和生物处理中的使用变得困难。此外，为了将缓冲流产生的压缩压降降到最小，介质的颗粒尺寸需要很大(50-100？直径)。大分子在颗粒孔内的大颗粒尺寸和小扩散系数降低了分离过程的效率。该项目的潜在创新解决了现有技术的固有问题。这项研究将为重组蛋白和生物大分子的纯化和分离提供新的、低成本的材料和技术。该项目的潜在商业应用是：(A)用于阴阳离子交换层析的非织造布涂层材料，(B)用于亲和层析的非织造布涂层材料，(C)用于放大层析的低成本一次性设备，以及(D)对涉及膜产品的公司的特定应用许可证。拟议的研究与公共卫生的相关性是为了加速和改进正在开发的治疗药物的开发，这些药物正在开发中，以消除癌症造成的痛苦和死亡。来自该项目的技术需要确保交付，并降低大量需要的新的抗癌疗法，如重组蛋白质的成本。

DESCRIPTION (provided by applicant): This project is focused on the development of a new type of chromatographic medium prepared by applying very thin polymeric coatings onto non-woven fabrics and having ion exchange groups suitable for adsorption and purification of large biomolecules. The resulting chromatographic media and devices will have a combination of high efficiency and robustness, making their use more effective, more efficient, and at lower cost than existing media for similar applications. The specific aims are to (1) optimize and develop chemistries for a family of coated non-wovens having ion exchange functionalities, (2) develop and demonstrate the performance of the coating process using an apparatus for continuous coating of non-woven sheet stock, (3) characterize substrates for applications to recombinant proteins, (4) build prototype devices based on the properties of the coated non-wovens and characterize performance. Purification of large biomolecules is an important component of biological research and for the production of recombinant proteins utilized for numerous applications in medicine. Conventional chromatographic media for this purpose have large pores to accommodate these large molecules. As a consequence, they are fragile and compressive making their use in column chromatography and bioprocessing difficult. Also, to minimize the compressive pressure drops produced by buffer flow, the particle size of the media need to be large (50-100 ? dia.). The large particle size and small diffusion coefficients of large molecules within the particle pores reduces efficiency of the separation process. Potential innovations of this project address the inherent problems with existing technology. This research will result in new, low cost materials and technology for purification and isolation of recombinant proteins and large biomolecules. Potential commercial applications of this project are (a) coated non-woven materials for anion and cation exchange chromatography, (b) coated non-woven materials for affinity chromatography, (c) low cost, disposable devices for scale-up chromatography, and (d) application specific licenses to companies involved in membrane products. The relevance of the proposed research to public heath is for the accelerated and improved development of therapeutic drugs that are being developed to eliminate suffering and death due to cancer. The technology...from this project is needed to ensure delivery and reduce the cost of new, anti-cancer therapeutics such as recombinant proteins which are needed in large quantities.