NOVEL UNIFORMLY SPACED VERY LARGE DNA SIZE MARKERS

需要均匀间隔的双链DNA大小标准品（梯） 具有在40个内切酶对（kbp）至10个内切酶对（kbp）范围内的不同间距 兆碱基对（Mbp）及以上，用于脉冲场凝胶电泳。 可商购的大小标记物（例如，DNA梯、酵母 染色体标记）是不够的。 我们建议制定一项补充性的 一组新的DNA梯，间距为40 kbp至约760 kbp， 范围从40 kbp到约10 Mbp（或更高）， 技术.

There is a need for uniformly spaced duplex DNA size standards (ladders) with different spacings in the range of 40 kilobase-pairs (kbp) to 10 megabase-pairs (Mbp) and above for use in pulsed field gel electrophoresis. Commercially available size markers (e.g. lambda-DNA ladders, yeast chromosomal markers) are inadequate. We propose to develop a complementary set of novel DNA ladders with spacings of 40 kbp to about 760 kbp and ranging from 40 kbp to about 10 Mbp (or higher) by using a patent-pending technology.