MAGNETICALLY STABILIZED CELL CULTURE SYSTEM

这项研究计划的总体目标是开发成本- 高效细胞培养技术在中药生产中的应用 单抗或治疗性蛋白等产品。 该方法涉及细胞在磁性材料上的生长。 敏化微载体珠，使得更高的微载体 密度，这又意味着更高的细胞密度(大于1.0 X_(107)/毫升)可用于生物反应器。这样做的其他好处 方法包括与现有文化的硬性兼容和 程序、易于扩展、简化控制和新功能 优化细胞新陈代谢和产品产量的机会。 第一阶段的研究目标是将其发展成磁性的 敏化微载体并展示其潜在的用途 锚定依赖细胞的细胞培养。这项研究还将 研究不同涂层材料及其与细胞的相互作用 依恋和超脱。在没有氧气限制的情况下 或中等耗竭，第一阶段的研究将证明 实验室在磁性条件下可达到的最大细胞密度 稳定的生物反应器系统。第二阶段研究将延长和 将这些结果放大到商业相关的细胞培养 条件。

The overall objective of this research program is to develop cost- effective cell culture technology for the manufacturing of products such as monoclonal antibodies or therapeutic proteins. The approach involves growth of cells on a magnetically sensitized microcarrier bead so that much higher microcarrier density, which in turn means higher cell density (greater than 1.0 x 107/ml) can be used in a bioreactior. Other advantages of this approach include compatibility with existing culture hardward and procedures, ease of scale-up, simplified control, and new opportunities to optimize cellular metabolism and product yield. Phase I research objective is to develop this magnetically sensitized microcarriers and demonstrate its potential utility for cell culture of anchorage dependent cells. This research will also study various coating materials and their interactions with cell attachment and detachment. In the absence of oxygen limitation or medium exhaustion, the Phase I research will demonstrate the maximum achievable cell density in a laboratory magnetically stabilized bioreactor system. Phase II research will extend and scale up these results to commercially relevant cell culture conditions.