PICOLITER LASER-BASED ABSORBANCE AND RI DETECTOR

毛细管分离技术对以下方面提出了严格的要求： 色谱检测系统。 分离检测器 技术，如毛细管液相色谱法、毛细管 超临界流体色谱和毛细管区 电泳，需要测量微量的 分析物。 探测器必须具有亚纳升的单元体积，以避免 效率和分辨率损失由于溶质带宽。 目前，具有高灵敏度和低成本的低容量检测器被广泛使用。 保持高效分离的完整性， 市售。 热光吸收测量可以 可用于进行非常灵敏的吸光度测定， 皮升体积的细胞。 拟议的研究将在 毛细管热光吸收检测技术 层析 在第一阶段，基于皮升激光的 吸光度/折射率（RI）检测器，用于高灵敏度低 基于毛细管的流动系统中的体积分析物检测将 追究 在第二阶段，探测器将与 性能评价用毛细管色谱系统 综合分析系统旨在阐明 生物分子和细胞的结构和功能。 总目标 的建议是开发一个商业皮升体积， 用于毛细管色谱系统的热光检测器。

Capillary separation techniques place stringent requirements on chromatographic detection systems. Detectors for separation techniques, such as capillary liquid chromatography, capillary supercritical fluid chromatography, and capillary zone electrophoresis, are required to measure minute amounts of the analytes. Detectors must have sub-nanoliter cell volumes to avoid efficiency and resolution loss due to solute band-broadening. Currently, low volume detectors that have high sensitivity and retain the integrity of the highly efficient separation are not commercially available. Thermo-optical absorbance measurements can be used to make very sensitive absorbance determinations within picoliter volume cells. The proposed research will develop on thermo-optical absorbance technique for detection in capillary chromatography. In Phase I, the feasibility of the picoliter laser-based absorbance/refractive index (RI) detector for highly sensitive low volume analyte detection in capillary based flowing systems will be investigated. In Phase II, the detector will be interfaced to capillary chromatographic systems for performance evaluations of the integrated system for analyses aimed at elucidating biomolecular and cellular structure and function. The overall goal of the proposal is to develop a commercial picoliter volume, thermo-optical detector for capillary chromatographic systems.