PICOLITER LASER-BASED ABSORBANCE AND RI DETECTOR

毛细管分离技术对 层析检测系统。分离用检测器 技术，如毛细管液相色谱、毛细管 超临界流体色谱和毛细管区带 电泳法，需要测量微量的 分析物。探测器必须具有亚纳升细胞体积，以避免 由于溶质带展宽而造成的效率和分辨率损失。 目前，具有高灵敏度和低容量的检测器 保持完整性的高效分离是不可能的 商业上可以买到的。热光吸收测量可以 用于进行非常灵敏的吸光度测定 皮升体积细胞。拟议的研究将在以下方面发展 热光吸收技术在毛细管检测中的应用 层析法。 在第一阶段，皮升激光的可行性 高灵敏度Low的吸光度/折射率(RI)检测器 毛细管流动系统中的体积分析物检测将 被调查。在第二阶段，探测器将连接到 用于性能评价的毛细管色谱系统 以澄清为目的的综合分析系统 生物分子和细胞的结构和功能。总目标 该提案的一部分是开发商业皮升容量， 用于毛细管色谱系统的热光检测器。

Capillary separation techniques place stringent requirements on chromatographic detection systems. Detectors for separation techniques, such as capillary liquid chromatography, capillary supercritical fluid chromatography, and capillary zone electrophoresis, are required to measure minute amounts of the analytes. Detectors must have sub-nanoliter cell volumes to avoid efficiency and resolution loss due to solute band-broadening. Currently, low volume detectors that have high sensitivity and retain the integrity of the highly efficient separation are not commercially available. Thermo-optical absorbance measurements can be used to make very sensitive absorbance determinations within picoliter volume cells. The proposed research will develop on thermo-optical absorbance technique for detection in capillary chromatography. In Phase I, the feasibility of the picoliter laser-based absorbance/refractive index (RI) detector for highly sensitive low volume analyte detection in capillary based flowing systems will be investigated. In Phase II, the detector will be interfaced to capillary chromatographic systems for performance evaluations of the integrated system for analyses aimed at elucidating biomolecular and cellular structure and function. The overall goal of the proposal is to develop a commercial picoliter volume, thermo-optical detector for capillary chromatographic systems.