CONTRIBUTE SURFACE PROPERTIES--ANTIBODY IMMOBILIZATION

贡献表面特性——抗体固定化

• 批准号: 3507832
• 负责人: SUSAN J MROCZKOWSKI
• 金额: $ 22.26万
• 依托单位: MIDWEST RESEARCH TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3507832
• 关键词: antibody; biomedical equipment resource; clinical biomedical equipment; immunopathology diagnosis; surface property

The immobilization of proteins on insoluble surfaces is important in diagnostic instrumentation and in vivo monitoring. Improvements in the surface antibody interactions should result in more efficient approaches to immunodiagnostics. This would be especially useful in the field of tumor marker detection where panels of antigens may be needed for proper diagnosis. The proposed research under Phase II is the development of a multiple immunological test with an electronic endpoint. The research will involve the use of antigen or antibody-labeled colloidal gold particles to generate an electronic endpoint. A diagnostic element will be refined so that the antibody-antigen reaction causes the completion of an open electrical circuit. This model necessitates an electrically insulating base surface for protein adhesion. A pair of conductors is placed on the base defining a channel between them. The antigen is placed in the channel. The gold-tagged antibody will bond to the specific antigen forming a conductive bridge across the channel for a positive reaction. This new type of device could replace the currently used enzyme immunoassay, immunofluorescence and radioimmunoassay techniques which are reliable but are slow, tedious, and restricted to single tests per sample. The commerical applications of a multiple test are broad, encompassing human diagnostics such as cancer and allergy as well as veterinary and forensic diagnostics.

将蛋白质固定在不溶性表面上很重要 诊断仪器和体内监测。 表面抗体相互作用的改进应导致 更有效的免疫诊断方法。 这将是 特别适用于肿瘤标志物检测领域 正确诊断可能需要一组抗原。 第二阶段拟议的研究是开发 具有电子终点的多重免疫学测试。 该研究将涉及使用抗原或抗体标记 胶体金颗粒产生电子端点。 一个 诊断要素将被细化，以便抗体-抗原 反应导致形成开路电路。 该模型需要一个电绝缘的基面 用于蛋白质粘附。 一对导体放置在底座上 定义它们之间的通道。 将抗原置于 渠道。 金标记的抗体将结合到特定的 抗原在通道上形成导电桥 阳性反应。 这种新型装置可以取代目前使用的酶 免疫分析、免疫荧光和放射免疫分析 可靠但缓慢、乏味的技术 仅限于每个样品的单一测试。 商业化的 多重测试的应用非常广泛，涵盖人类 癌症和过敏等诊断以及兽医和 法医诊断。