CONTRIBUTION OF SURFACE PROPERTIES TO AB IMMOBILIZATION

蛋白质在不溶性表面的固定化是重要的 用于诊断仪器和体内监测。 表面抗体相互作用的改善应导致 更有效的免疫诊断方法。 这将是 特别适用于肿瘤标志物检测领域， 可能需要一组抗原用于正确诊断。 第二阶段的拟议研究是开发一种 多项免疫学测试，电子终点。 这项研究将涉及使用抗原或抗体标记的 胶体金颗粒以产生电子端点。 一 诊断元件将被改进， 反应导致开路的完成。 该模型需要电绝缘的基底表面 用于蛋白质粘附。 一对导体放置在底座上 在它们之间形成通道。 将抗原置于 频道 金标记的抗体会结合到 抗原形成跨通道的导电桥， 积极的反应。 这种新型设备可以取代目前使用的酶 免疫测定、免疫荧光和放射免疫测定 技术可靠，但缓慢，繁琐， 仅限于对每个样本进行单次测试。 《圣经》 多重测试的应用是广泛的，包括人类 癌症和过敏等诊断以及兽医和 法医诊断。

The immobilization of proteins on insoluble surfaces is important in diagnostic instrumentation and in vivo monitoring. Improvements in the surface antibody interactions should result in more efficient approaches to immunodiagnostics. This would be especially useful in the field of tumor marker detection where panels of antigens may be needed for proper diagnosis. The proposed research under Phase II is the development of a multiple immunological test with an electronic endpoint. The research will involve the use of antigen or antibody-labeled colloidal gold particles to generate an electronic endpoint. A diagnostic element will be refined so that the antibody-antigen reaction causes the completion of an open electrical circuit. This model necessitates an electrically insulating base surface for protein adhesion. A pair of conductors is placed on the base defining a channel between them. The antigen is placed in the channel. The gold-tagged antibody will bond to the specific antigen forming a conductive bridge across the channel for a positive reaction. This new type of device could replace the currently used enzyme immunoassay, immunofluorescence and radioimmunoassay techniques which are reliable but are slow, tedious, and restricted to single tests per sample. The commerical applications of a multiple test are broad, encompassing human diagnostics such as cancer and allergy as well as veterinary and forensic diagnostics.