CARCINOEMBRYONAL ANTIGEN IDIOTYPE VACCINE

描述：(改编自申请者摘要)癌症患者 通常对肿瘤细胞上表达的抗原具有耐受性。 触发患者对肿瘤相关的主动免疫反应 抗原代表了癌症最有希望的前景之一。 心理治疗。在这个项目中，研究人员提出了一项临床前研究 触发主动免疫反应的抗独特型方法 对CEA的指控。某些抗独特型抗体针对 针对特定抗原的抗体的可变区可以模仿 (即，带有该抗原的内部图像)。在几个实验中 系统，用抗独特型抗体免疫可引起特异性 免疫代替原始抗原的免疫力。用抗-DNA免疫 独特型抗体作为替代抗原是一种很有前途的方法 触发对耐受抗原的免疫，如肿瘤抗原。他们有 已产生的狒狒多克隆和大鼠单克隆抗独特型抗体 抗原性模仿CEA的抗体。在这个项目中，他们将 评估这些抗独特型抗体是否可以作为替代品 仓鼠体内的抗原。仓鼠之所以被选中，是因为它们是唯一 已知的非免疫缺陷动物物种，在这些物种中人类产生CEA 肿瘤可能会生长。仓鼠代表了一种独特的模型来测试是否活跃 抗独特型免疫可防止肿瘤生长。这一阶段 我的研究将为更广泛的临床前和 第二阶段的临床研究。

DESCRIPTION: (Adapted from the applicant's abstract) Cancer patients are usually tolerant to the antigens expressed on tumor cells. Triggering an active immune response by the patients to tumor-associated antigens represents one of the most promising prospects in cancer therapy. In this project, the investigators propose a preclinical study of the anti-idiotype approach to trigger an active immune response against CEA. Certain anti-idiotypic antibodies directed against the variable regions of antibodies specific for a given antigen can mimic (i.e., bear the internal image of) this antigen. In several experimental systems, immunization with anti-idiotypic antibodies elicited specific immunity in lieu of the original antigen. Immunization with anti- idiotype antibodies as surrogate antigens is a promising approach to trigger immunity to tolerized antigens such as tumor antigens. They have already generated baboon polyclonal and rat monoclonal anti-idiotype antibodies that antigenically mimic CEA. In this project they will assess whether these anti-idiotype antibodies can function as surrogate antigens in hamsters. Hamsters have been chosen since they are the only known non-immunodeficient animal species in which human CEA-producing tumors may grow. Hamsters represent a unique model to test whether active anti-idiotype immunization can protect against tumor growth. This Phase I study will represent the basis for more extensive preclinical and clinical studies in Phase II.