LARGE METAL CLUSTER AND CLUSTER-POLYMER IMMUNOPROBES

大金属簇和簇聚合物免疫探针

• 批准号: 3498970
• 负责人: RICHARD DENIS POWELL
• 金额: $ 5万
• 依托单位: NANOPROBES, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-01 至 1993-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3498970
• 关键词: chemical synthesis; covalent bond; electron density; gold; hybrid antibody; hydropathy; immunoconjugates; immunoelectron microscopy; ligands; monomer; palladium; platinum; polymers

New, molecular heavy metal cluster immunoprobes will be prepared, with platinum and palladium clusters 1.8 and 2.4 nm in diameter covalently conjugated to antibodies to give immunoelectron microscopy probes. Multiple gold cluster complexes will also be combined in a single larger label, up to 5 nm in diameter, using starburst dendrimer molecules as carriers for undecagold and a larger 1.4 nm gold cluster complex. Syntheses are proposed for new dendrimers based on adamantylic initiator cores and chain branching monomers, and rigid rod spacers: these will be also be used as single-label carriers for multiple gold clusters. The resulting probes should combine the ease of visibility of larger colloidal gold particles with high sensitivity, low non-specific protein affinity, no aggregation or label dissociation, and penetration up to 30 micro into cells and tissue sections. New multiple gold cluster probes used with silver enhancement in antigen detection blots, should demonstrate unprecedented sensitivities, possibly an order of magnitude or more higher than current chemiluminescence methods. New dendrimers are proposed which incorporate gold clusters into the constituent monomers: these will be used as precisely defined immunoprobe labels up to 10 nm in diameter, and will also be studied as potential precursors to new classes of materials.

将制备新的分子重金属簇免疫探针， 直径分别为1.8和2.4 nm的铂和钯簇 与抗体偶联，得到免疫电子显微镜探针。 多个金簇复合物也将组合在一个更大的 标记，直径高达5 nm，使用星状树状聚合物分子作为 载体的undecagold和更大的1.4 nm的金簇复合物。 提出了基于金刚烷基引发剂的新型树枝状大分子的合成方法 核和链支化单体以及刚性棒间隔物：这些将 也可用作多个金簇的单标记载体。 的 由此产生的探头应结合联合收割机， 胶体金颗粒具有高灵敏度、低非特异性蛋白 亲和性，无聚集或标记解离，渗透率高达30 显微镜下的细胞和组织切片。 新型多金簇探针 与银增强一起用于抗原检测印迹，应 显示出前所未有的敏感性， 或比目前的化学发光方法更高。 新型树枝状大分子 提出了将金团簇结合到组分中， 单体：这些将用作精确定义的免疫探针标记 直径为10 nm，也将作为潜在的前体进行研究 到新的材料类别。