RAPID CHEMILUMINESCENT ASSAY FOR LUTEINIZING HORMONE

黄体生成素的快速化学发光测定

• 批准号: 3499704
• 负责人: JOHN C VOYTA
• 金额: $ 5万
• 依托单位: TROPIX, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1990-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3499704
• 关键词: alkaline phosphatase; antiantibody; chemical conjugate; fertility; gel electrophoresis; high performance liquid chromatography; immunologic techniques; luminescence; luteinizing hormone; monoclonal antibody; ovulation; oxetane; photography; urinalysis

In this application, a proposal to generate a rapid chemiluminescence based immunoassay for urinary luteinizing hormone is described. A prototype system based on a membrane supported, two-site sandwich immunoassay, with an alkaline phosphatase labeled detector antibody will be developed. Incorporation of 3- (2'adamantane)-4-(3" phosphoryloxy)phenyl-1,2-dioxetane, (AMPPD), will be investigated. Dephosphorylation of AMPPD with alkaline phosphatase results in a glow of light which will be detected with Polaroid instant film. Peak LH levels will be detected as a white spot on the film, in contrast to black or gray spots imaged when LH concentration is low. The film-based results will provide the user a permanent record which will facilitate day to day comparisons, for both patients and their physician. Phase II of this program will involve the design and engineering of a disposable unidose device and a prototype of a disposable imaging instrument. This LH assay kit will then be tested with clinically relevant LH containing specimen.

在本申请中，提出了一种产生快速化学发光的方法， 本发明描述了一种基于免疫测定法的尿促黄体生成素的检测方法。一 基于膜支撑的双位点夹层结构的原型系统 使用碱性磷酸酶标记检测抗体的免疫测定将 发展。3-（2 '金刚烷）-4-（3” 将研究磷酰氧）苯基-1，2-二氧杂环丁烷（AMPPD）。 AMPPD与碱性磷酸酶的去磷酸化导致 宝丽来即时胶片可以检测到的光线。LH峰值水平 将被检测为胶片上的白色斑点，与黑色或 当LH浓度低时成像的灰点。基于电影的结果 将为用户提供一个永久的记录，这将有助于每天 比较，为患者和他们的医生。 该计划的第二阶段将涉及设计和工程的一个 一次性单剂量装置和一次性成像装置的原型 仪器该LH检测试剂盒将在临床上进行测试， 含LH的相关标本。