CHEMI-LUMINESCENT DETECTION OF LUTEINIZING HORMONE

黄体生成素的化学发光检测

• 批准号: 3508252
• 负责人: JOHN C VOYTA
• 金额: $ 34.15万
• 依托单位: TROPIX, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3508252
• 关键词: alkaline phosphatase; antibody formation; bioluminescence; enzyme linked immunosorbent assay; human; immunologic assay /test; luteinizing hormone; radioimmunoassay; serum; urine

In this application, a proposal to generate a rapid chemiluminescence based immunoassay for urinary luteinizing hormone is described. A prototype system based on a membrane supported, two-site sandwich immunoassay, with an alkaline phosphatase labeled detector antibody will be developed. Incorporation of 3- (2'adamantane)-4-(3" phosphoryloxy)phenyl-1,2-dioxetane, (AMPPD), will be investigated. Dephosphorylation of AMPPD with alkaline phosphatase results in a glow of light which will be detected with Polaroid instant film. Peak LH levels will be detected as a white spot on the film, in contrast to black or gray spots imaged when LH concentration is low. The film-based results will provide the user a permanent record which will facilitate day to day comparisons, for both patients and their physician. Phase II of this program will involve the design and engineering of a disposable unidose device and a prototype of a disposable imaging instrument. This LH assay kit will then be tested with clinically relevant LH containing specimen.

在本申请中，一种产生快速化学发光的提议 本文介绍了尿液黄体生成素的免疫分析方法。一个 基于膜支承双部位夹层的原型系统 免疫分析，用碱性磷酸酶标记的抗体检测 被开发出来。3-(2‘金刚烷)-4-(3“)的引入 将对苯基-1，2-二氧杂环己烷(AMPPD)进行研究。 碱性磷酸酶对AMPPD的去磷酸化导致发光 用宝丽来瞬间胶片可以探测到的光线。黄体生成素峰值水平 将被检测为胶片上的白色斑点，而不是黑色或 当黄体生成素浓度较低时，可见灰斑点。以电影为基础的结果 将为用户提供一个永久的记录，以方便日常工作 对病人和他们的医生来说都是比较。 该计划的第二阶段将涉及设计和工程 一次性统一设备和一次性成像的原型 乐器。此黄体生成素检测试剂盒随后将进行临床测试 含黄体生成素的相关标本。