FLOW/STERIC FFF--A NEW METHOD FOR CELL SEPARATIONS

流动/空间 FFF——细胞分离的新方法

• 批准号: 3501271
• 负责人: BHAJENDRA N BARMAN
• 金额: $ 5万
• 依托单位: FFFRACTIONATION, LLC
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3501271
• 关键词: blood disorder diagnosis; blood fractionation; cell sorting; erythrocytes; fluid flow; human tissue; morphology; particle counter

Flow/Steric Field-Flow Fractionation (flow/StFFF) is a recently developed method with extraordinary speed and resolving power designed to separate particulate materials falling roughly in the 1-100 um particle diameter range. The object of this proposal is to examine the potential of converting this research finding into a fast and reliable tool for the fractionation and characterization of cells in samples of clinical and biochemical interest. In flow/StFFF sample particles are subjected to a crossflow field while being carried along by a carrier liquid in a ribbon-like thin channel with permeable walls. Cell particles will elute depending on their size and shape, and in some cases, density. As these parameters are reflected in the elution profiles or fractograms, one should be able to identify the difference between normal and abnormal cells by observing the pattern of the fractograms. This method will be studied for its possible routine application to cell populations. In particular, we will examine its applicability to the diagnosis of abnormal human red blood cells. The ability of flow/StFFF to isolate pure cell populations of viable cells will also receive major attention. The proposed method, once developed, should be relatively simple and cost effective. The analysis time with this method is now approximately 1-5 minutes for a sample, with additional reduction possible. From 10.5 to 10.7 cells are expected t constitute the optimum sample size.

流动/空间场-流动分级（Flow/StFFF）是近年来发展起来的一种新的分离技术。 一种速度和分辨率都非常高的新方法 设计用于分离大致落入 1-100 μ m粒径范围。 这项建议的目的是 来研究将这项研究成果转化为 一种快速可靠的分离和表征工具 临床和生物化学感兴趣的样品中的细胞。 在流动/StFFF中，样品颗粒经受交叉流场 同时被载液沿着以带状薄的形式携带， 具有可渗透壁的通道。 细胞颗粒将根据 它们的大小和形状，在某些情况下，密度。 因为这些 参数反映在洗脱分布图或分形图中， 应该能够区分正常和 通过观察碎片图的图案来识别异常细胞。 这 方法将研究其可能的常规应用于细胞 人口。 特别是，我们将研究其适用性， 诊断异常的人类红细胞。 的能力 flow/StFFF分离活细胞的纯细胞群将 也受到了极大的关注。 所提出的方法一旦发展起来，应该是相对简单的 和成本效益。 使用此方法的分析时间为 样品约1-5分钟，并进一步减少 可能 预期10.5至10.7个细胞构成 最佳样本量

项目成果

Separation and size distribution of red blood cells of diverse size, shape, and origin by flow/hyperlayer field-flow fractionation.

• DOI: 10.1006/abio.1993.1287
• 发表时间: 1993-07
• 期刊: Analytical biochemistry
• 影响因子: 2.9
• 作者: B. N. Barman;E. Ashwood;J. Giddings