PRECAST GELS--EXPERIMENTAL ADVANCE IN DNA SEQUENCING

预制凝胶——DNA 测序的实验进展

• 批准号: 3500445
• 负责人: GEORGE T LEKA
• 金额: $ 5万
• 依托单位: JULE BIOTECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3500445
• 关键词: DNA; biotechnology; gel; gel electrophoresis; reagent /indicator

While identification of the nucleotide sequence of deoxyribonucleic acid (DNA) is critical for the analysis and understanding of genetic information, the feasibility of this undertaking is limited by the experimental advances that are currently available. The importance of decoding the human genome are well known and exceed the scope of this proposal. Determination of the nucleotide sequence in DNA is an extraordinary task considering the large number of nucleotide units found in even the smallest DNA molecule. A highly reproducible, readily available vehicle for electrophoretic separation of the nucleotide bases in DNA is essential in order to achieve a significant advancement in this area of research. Commercially available precast gels for sequencing once considered a luxury, have become a necessity for the researcher. The objective of this proposal is to; develop a precast DNA sequencing gel, a process for pouring the gels and to perform testing of the gels in a university research lab. Phase II of this project will involve quality testing and addressing the problems of production scale-up with the ultimate goal of supplying a high quality and economical commercially available gel compatible with currently available DNA sequencers.

在鉴定脱氧核糖核酸的核苷酸序列时， (DNA)对于分析和理解遗传 信息，这项工作的可行性受到限制， 目前可用的实验进展。 的重要性 解码人类基因组是众所周知的，并超出了本发明的范围。 提议 确定DNA中的核苷酸序列是一种 这是一项非常艰巨的任务，因为我们发现了大量的核苷酸单位， 即使是最小的DNA分子。 一个高度可重复的，容易 用于电泳分离核苷酸碱基的可用载体， 为了在这一领域取得重大进展，DNA是必不可少的 的研究。 市售预制凝胶，用于一次测序 被认为是奢侈品，已经成为研究人员的必需品。 本提案的目标是：开发预制DNA测序凝胶， 一种用于倾倒凝胶并在一个或多个容器中进行凝胶测试的方法， 大学研究实验室 该项目的第二阶段将涉及质量 测试和解决生产规模扩大的问题， 最终目标是提供高质量和经济的商业 与目前可用的DNA测序仪相容的可用凝胶。