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DEVELOPMENT OF A QUANTITATIVE ANTIBODY BINDING KIT

定量抗体结合试剂盒的开发

基本信息

批准号：
3506705
负责人：
Abe Schwartz
金额：
$ 13.34万
依托单位：
CARIBBEAN MICROPARTICLES CORPORATION
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-09-01 至 1992-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3506705
关键词：
antigen antibody reaction cell population study chemical binding diagnosis quality /standard flow cytometry fluorescent dye /probe human tissue immunofluorescence technique immunological substance immunopathology diagnosis monoclonal antibody reagent /indicator

项目摘要

The feasibility of producing a set of microbeads (Quantitative Simply Cellular Calibration Kit) which can be used with a flow cytometer to directly quantitate the number of mAbs binding to specific populations of cells has been demonstrated. The objectives of this proposal are to improve the binding reactivity of the QSC microbeads, evaluate new methods of calibrating the binding sites of the microbeads and determine the precision and accuracy of measuring mABs binding to cells by this new methodology. An instruction manual and computerized analysis worksheet will be developed to support this kit. The QSC Calibration Kit and support materials will be initially evaluated by three outside investigators, followed by a more general evaluation via a workshop and sample distribution at the Clinical Cytometry Meeting in Charleston, SC. The development and commercialization of this methodology will provide clinical and research laboratories with a powerful and innovative diagnostic tool. Being able to quickly and accurately determine the number of mAbs binding to specific cell populations will help pathologists, immunologists and oncologists to achieve more accurate diagnosis, treatment and prognosis of immune related diseases, such as AIDS.

生产一套微珠（定量简单）的可行性