QUANTITATIVE INDO-1 MICROBEAD STANDARD IN FLOW CYTOMETRY

流式细胞术中的定量 INDO-1 微珠标准品

基本信息

  • 批准号:
    3498390
  • 负责人:
  • 金额:
    $ 5万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1989
  • 资助国家:
    美国
  • 起止时间:
    1989-07-01 至 1989-12-31
  • 项目状态:
    已结题

项目摘要

Intracellular calcium concentration is known to play a significant role in both physiological and pathophysiological conditions. Recently, much attention has been given to the determination of free intracellular calcium concentration using flow cytometry. Newly developed fluorescent probes, such as Indo-1 which exhibits a large spectral emission shift when it chelates Ca++ ions have been instrumental in the development of this application. However, because calibration of the flow cytometer response with respect to the ratio of the FL1 (405 nm peak) and the FL2 (485 nm peak) cytometry has been limited. The present proposal focuses on the development of an Indo-1 Microbead Standards Kit consisting of Indo-1 coated microbeads suspended in a series of solutions containing pre-determined concentrations of free Ca++. Using this kit the ratio of the FL1 and FL2 emission fluorescence of Indo-1 microbeads can be directly correlated with free calcium concentration. Such a kit will not only make calibration of flow cytometers more convenient, but will also help to normalize data over time and among laboratories. A more accurate determination of cytosolic calcium concentration will undoubtedly improve our understanding of the biological relevance of alterations in intracellular calcium concentration.
已知细胞内钙浓度起重要作用

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Abe Schwartz其他文献

Abe Schwartz的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Abe Schwartz', 18)}}的其他基金

NEW EGFP CALIBRATION STANDARDS FOR FLOW CYTOMETRY
适用于流式细胞术的新 EGFP 校准标准
  • 批准号:
    2874335
  • 财政年份:
    1999
  • 资助金额:
    $ 5万
  • 项目类别:
DEVELOPMENT OF A QUANTITATIVE ANTIBODY BINDING KIT
定量抗体结合试剂盒的开发
  • 批准号:
    3506705
  • 财政年份:
    1988
  • 资助金额:
    $ 5万
  • 项目类别:
DEVELOPMENT OF A QUANTITATIVE ANTIBODY BINDING KIT
定量抗体结合试剂盒的开发
  • 批准号:
    3506704
  • 财政年份:
    1988
  • 资助金额:
    $ 5万
  • 项目类别:
DEVELOPMENT OF A QUANTITATIVE ANTIBODY BINDING KIT
定量抗体结合试剂盒的开发
  • 批准号:
    3492070
  • 财政年份:
    1988
  • 资助金额:
    $ 5万
  • 项目类别:

相似海外基金

CAREER: Next-Generation Flow Cytometry - A New Approach to Cell Heterogeneity
职业:下一代流式细胞术 - 细胞异质性的新方法
  • 批准号:
    2422750
  • 财政年份:
    2024
  • 资助金额:
    $ 5万
  • 项目类别:
    Standard Grant
BioSynth Trust: Developing understanding and confidence in flow cytometry benchmarking synthetic datasets to improve clinical and cell therapy diagnos
BioSynth Trust:发展对流式细胞仪基准合成数据集的理解和信心,以改善临床和细胞治疗诊断
  • 批准号:
    2796588
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
    Studentship
Omics, Bioinformatics and Flow Cytometry Core
组学、生物信息学和流式细胞术核心
  • 批准号:
    10630580
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
Flow Cytometry Core
流式细胞术核心
  • 批准号:
    10630389
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
High-parametric flow cytometry cell sorter for human immunology studies.
用于人类免疫学研究的高参数流式细胞仪细胞分选仪。
  • 批准号:
    10632888
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
Time-lapse Flow Cytometry for Kinetic Profiling of T-Cell Function
用于 T 细胞功能动力学分析的延时流式细胞术
  • 批准号:
    10699148
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
Establishment of diagnostic method for pleural effusion in malignant pleural mesothelioma by flow cytometry focusing on cell cycle
以细胞周期为中心的流式细胞术诊断恶性胸膜间皮瘤胸腔积液的方法的建立
  • 批准号:
    23K15563
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Spectral Configured Bigfoot Sorter for Salk Institute Flow Cytometry Core
用于索尔克研究所流式细胞术核心的光谱配置 Bigfoot 分选机
  • 批准号:
    10632950
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
Flow Cytometry Core
流式细胞术核心
  • 批准号:
    10628881
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
Development and Clinical Application of a Novel Method for Simultaneous Assay of Antibody Drugs Using Flow Cytometry
流式细胞术同时测定抗体药物新方法的开发及临床应用
  • 批准号:
    23K06277
  • 财政年份:
    2023
  • 资助金额:
    $ 5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了