QUANTITATIVE INDO-1 MICROBEAD STANDARD IN FLOW CYTOMETRY
流式细胞术中的定量 INDO-1 微珠标准品
基本信息
- 批准号:3498390
- 负责人:
- 金额:$ 5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1989
- 资助国家:美国
- 起止时间:1989-07-01 至 1989-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Intracellular calcium concentration is known to play a significant role
in both physiological and pathophysiological conditions. Recently, much
attention has been given to the determination of free intracellular
calcium concentration using flow cytometry. Newly developed fluorescent
probes, such as Indo-1 which exhibits a large spectral emission shift
when it chelates Ca++ ions have been instrumental in the development of
this application. However, because calibration of the flow cytometer
response with respect to the ratio of the FL1 (405 nm peak) and the FL2
(485 nm peak) cytometry has been limited. The present proposal focuses
on the development of an Indo-1 Microbead Standards Kit consisting of
Indo-1 coated microbeads suspended in a series of solutions containing
pre-determined concentrations of free Ca++. Using this kit the ratio of
the FL1 and FL2 emission fluorescence of Indo-1 microbeads can be
directly correlated with free calcium concentration. Such a kit will not
only make calibration of flow cytometers more convenient, but will also
help to normalize data over time and among laboratories. A more accurate
determination of cytosolic calcium concentration will undoubtedly improve
our understanding of the biological relevance of alterations in
intracellular calcium concentration.
已知细胞内钙浓度起重要作用
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Abe Schwartz其他文献
Abe Schwartz的其他文献
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{{ truncateString('Abe Schwartz', 18)}}的其他基金
NEW EGFP CALIBRATION STANDARDS FOR FLOW CYTOMETRY
适用于流式细胞术的新 EGFP 校准标准
- 批准号:
2874335 - 财政年份:1999
- 资助金额:
$ 5万 - 项目类别:
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